Document Detail

Methods used for the isolation, enumeration, characterisation and identification of Enterococcus spp. 1. Media for isolation and enumeration.
MedLine Citation:
PMID:  14596987     Owner:  NLM     Status:  MEDLINE    
Due to their significance in food, feed, environmental and clinical samples, the detection and enumeration of enterococci have become an important issue not only in daily routine but also in current research activities. Several media and protocols have been published for diverse purposes, but there is no single method, which universally meets all requirements. Depending on the nature of the accompanying microflora and its level, certain substrates and modifications thereof have to be used, taking into account various drawbacks and advantages. In addition to the historical applications (examination of water, different kinds of foods, intestinal and other clinical specimen), the detection of vancomycin-resistant enterococci (VRE) has become an important task, since VRE have found to be frequently involved in nosocomial infections. Moreover, contradictory methodological recommendations can be found in the literature. This paper will give a systematic survey of the different media and methods proposed during the last two decades. Emphasis is placed on compositional details and on specific applications of the media described.
Konrad J Domig; Helmut K Mayer; Wolfgang Kneifel
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  International journal of food microbiology     Volume:  88     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-04     Completed Date:  2004-01-09     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  147-64     Citation Subset:  IM    
Department of Dairy Research and Bacteriology, BOKU-University of Natural Resources and Applied Life Sciences, Vienna, Gregor Mendel Strasse 33, A-1180 Vienna, Austria.
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MeSH Terms
Colony Count, Microbial / methods*
Culture Media / chemistry*
Enterococcus / drug effects,  growth & development,  isolation & purification*
Food Microbiology*
Vancomycin Resistance
Water Microbiology*
Reg. No./Substance:
0/Culture Media

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