Document Detail


Methods to identify and characterize different NK cell receptors and their ligands.
MedLine Citation:
PMID:  20033646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Different cellular immune responses are modulated by the cross talk between activating and inhibitory signaling pathways initiated via different cell surface receptors. Similarly, the killing of NK cells is controlled by multiple activating and inhibitory surface receptors. In humans, the major NK triggering receptors, identified so far, include NKp80, 2B4 NKG2D, and CD16 and the natural cytotoxic receptors (collectively named NCRs) include NKp46, NKp44, and NKp30. The two major families of MHC-specific inhibitory receptors identified in humans are the Ig superfamily (KIR and LIR) and the C-type lectin (CD94/NKG2A) receptor superfamily. The different inhibitory receptors show diverse specificity and discriminate between different class I MHC proteins. Much is known about the function and expression patterns of the different NK cell receptors, but the ligand identity of several of the activating NK cell receptors is yet to be discovered. This chapter introduces several research tools that can be used to uncover the identities of different ligands for NK cell receptors.
Authors:
Dikla Lankry; Roi Gazit; Ofer Mandelboim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  612     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2009-12-24     Completed Date:  2010-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-73     Citation Subset:  IM    
Affiliation:
The Hebrew University - Hadassah Medical School, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD3 / metabolism
Cell Line
Cell Separation
Clone Cells
Electroporation
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation
Gene Knockout Techniques
Gene Targeting
Green Fluorescent Proteins / metabolism
Humans
Interleukin-2 / secretion
Killer Cells, Natural / cytology,  immunology,  metabolism
Ligands
Mice
Point Mutation
Receptors, Natural Killer Cell / analysis,  deficiency,  genetics,  metabolism*
Recombinant Fusion Proteins / deficiency,  genetics,  metabolism
Retroviridae / genetics
Transfection
Chemical
Reg. No./Substance:
0/Antigens, CD3; 0/CD3 antigen, zeta chain; 0/Interleukin-2; 0/Ligands; 0/Receptors, Natural Killer Cell; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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