Document Detail

Methods to detect biomarkers of cellular senescence: the senescence-associated beta-galactosidase assay.
MedLine Citation:
PMID:  17634571     Owner:  NLM     Status:  MEDLINE    
Most normal human cells undergo cellular senescence after accruing a fixed number of cell divisions, or are challenged by a variety of potentially oncogenic stimuli, in culture and most likely in vivo. Cellular senescence is characterized by an irreversible growth arrest and certain altered functions. Senescent cells in culture are identified by their inability to undergo DNA synthesis, a property also shared by quiescent cells. Several years ago, we described a biomarker associated with the senescent phenotype, a senescence associated beta-galactosidase (SA-beta-gal), which is detected by histochemical staining of cells using the artificial substrate X-gal. The presence of the SA-beta-gal biomarker is independent of DNA synthesis and generally distinguishes senescent cells from quiescent cells. The method to detect SA-beta-gal is a convenient, single cell-based assay, which can identify senescent cells even in heterogeneous cell populations and aging tissues, such as skin biopsies from older individuals. Because it is easy to detect, SA-beta-gal is currently a widely used biomarker of senescence. Here we describe a method to detect SA-beta-gal in detail, including some recent modifications.
Koji Itahana; Judith Campisi; Goberdhan P Dimri
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  371     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-09-21     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  21-31     Citation Subset:  IM    
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MeSH Terms
Aging / metabolism*
Biological Markers / analysis,  metabolism
Cell Aging / physiology*
Cell Line
DNA / biosynthesis
Fibroblasts / cytology,  enzymology*
Galactosides / chemistry
Indoles / chemistry
Skin / enzymology*
beta-Galactosidase / analysis,  metabolism*
Grant Support
R01 CA094150/CA/NCI NIH HHS; R01 CA094150-05/CA/NCI NIH HHS
Reg. No./Substance:
0/Biological Markers; 0/Galactosides; 0/Indoles; 9007-49-2/DNA; EC; V595OG374W/5-bromo-4-chloro-3-indolyl beta-galactoside

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