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Methods to Study Legionella Transcriptome In Vitro and In Vivo.
MedLine Citation:
PMID:  23150421     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The study of transcriptome responses can provide insight into the regulatory pathways and genetic factors that contribute to a specific phenotype. For bacterial pathogens, it can identify putative new virulence systems and shed light on the mechanisms underlying the regulation of virulence factors. Microarrays have been previously used to study gene regulation in Legionella pneumophila. In the past few years a sharp reduction of the costs associated with microarray experiments together with the availability of relatively inexpensive custom-designed commercial microarrays has made microarray technology an accessible tool for the majority of researchers. Here we describe the methodologies to conduct microarray experiments from in vitro and in vivo samples.
Authors:
Sebastien P Faucher; Howard A Shuman
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  567-582     Citation Subset:  -    
Affiliation:
Faculty of Agricultural and Environmental Sciences, Department of Natural Resource Sciences, McGill University, Quebec, Canada, sebastien.faucher2@mcgill.ca.
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