Document Detail


Methods for synchronizing cells at specific stages of the cell cycle.
MedLine Citation:
PMID:  18228388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exponentially growing cells are asynchronous with respect to the cell cycle stage. Detection of cell cycle-related events is improved by enriching the culture for cells at the stage during which the particular event occurs. Methods for synchronizing cells are provided here, including those based on morphological features of the cell (mitotic shake-off), cellular metabolism (thymidine inhibition, isoleucine depravation), and chemical inhibitors of cell progression in G1 (lovastatin), S (aphidicolin, mimosine), and G2/M (nocodazole). Applications of these methods and the advantages and disadvantages of each are described.
Authors:
J Jackman; P M O'Connor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]     Volume:  Chapter 8     ISSN:  1934-2616     ISO Abbreviation:  Curr Protoc Cell Biol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-01-29     Completed Date:  2008-02-20     Revised Date:  2009-12-11    
Medline Journal Info:
Nlm Unique ID:  101287856     Medline TA:  Curr Protoc Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 8.3     Citation Subset:  IM    
Affiliation:
U.S.A.M.R.I.I.D., Fort Dietrick, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aphidicolin / pharmacology
Cell Adhesion
Cell Cycle / drug effects*,  physiology
Cell Line, Tumor / cytology,  drug effects
Cell Separation / methods
Culture Media, Serum-Free / pharmacology
Humans
Isoleucine / metabolism
Lovastatin / pharmacology
Mice
Mimosine / pharmacology
Mitosis
Mitotic Index
Mitotic Spindle Apparatus / drug effects
NIH 3T3 Cells / cytology,  drug effects
Nocodazole / pharmacology
Thymidine / metabolism
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 31430-18-9/Nocodazole; 38966-21-1/Aphidicolin; 50-89-5/Thymidine; 500-44-7/Mimosine; 73-32-5/Isoleucine; 75330-75-5/Lovastatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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