Document Detail

Methods for studying the evolution of plant reproductive structures: comparative gene expression techniques.
MedLine Citation:
PMID:  15865987     Owner:  NLM     Status:  MEDLINE    
A major component of evolutionary developmental (evo-devo) genetics is the analysis of gene expression patterns in nonmodel species. This comparative approach can take many forms, including reverse-transcriptase polymerase chain reaction, Northern blot hybridization, and in situ hybridization. The choice of technique depends on several issues such as the availability of fresh tissue, as well as the expected expression level and pattern of the candidate gene in question. Although the protocols for these procedures are fairly standard, optimization is often required because of the specific characteristics of the species under analysis. This chapter describes several methods commonly used to determine gene expression patterns in angiosperms, particularly in floral tissues. Suggestions for adapting basic protocols for diverse taxa and troubleshooting are also extensively discussed.
Elena M Kramer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  395     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-05-03     Completed Date:  2005-08-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  617-36     Citation Subset:  IM    
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
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MeSH Terms
Blotting, Northern / methods
Gene Expression*
Genes, Plant
Genetic Techniques*
Histological Techniques
In Situ Hybridization / methods
Plant Physiological Phenomena
Plants / anatomy & histology,  genetics*
Polymerase Chain Reaction / methods
RNA Probes
RNA, Plant / genetics,  isolation & purification
Reg. No./Substance:
0/RNA Probes; 0/RNA, Plant

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