Document Detail


Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies.
MedLine Citation:
PMID:  15576935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The determination of structures for proteins that control the eukaryotic cell cycle by nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography has made a significant contribution to our understanding of the molecular mechanisms that control cell cycle progression. CDK2 has proved particularly tractable to structural analysis, and CDK2 in complex with various regulatory proteins and in different phosphorylation states provides a paradigm for the control of this important kinase family. This chapter describes a number of protocols that can be used to prepare CDKs and selected CDK binding proteins suitable for structural studies by heterologous expression in either E. coli or insect cells.
Authors:
Julie Welburn; Jane Endicott
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  296     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-03     Completed Date:  2005-03-29     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-35     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Oxford, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
CDC2-CDC28 Kinases / chemistry,  genetics,  isolation & purification
Cattle
Cell Cycle / physiology*
Cell Cycle Proteins / genetics,  isolation & purification*,  physiology
Cell Line
Crystallography, X-Ray
Cyclin A / chemistry,  genetics,  isolation & purification
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases / genetics,  isolation & purification,  physiology
Escherichia coli / genetics
Eukaryotic Cells
Humans
Models, Molecular
Multiprotein Complexes
Peptide Fragments / chemistry,  genetics,  isolation & purification
Phosphorylation
Recombinant Proteins / chemistry,  genetics,  isolation & purification
Spodoptera
Xenopus Proteins / chemistry,  genetics,  isolation & purification
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Cyclin A; 0/Multiprotein Complexes; 0/Peptide Fragments; 0/Recombinant Proteins; 0/Xenopus Proteins; 0/cyclin A3 protein, Bos taurus; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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