| Methods for nutrigenomics and longevity studies in Drosophila: effects of diets high in sucrose, palmitic acid, soy, or beef. | |
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MedLine Citation:
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PMID: 17634578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nutrigenomics is the study of gene-nutrient interactions and how they affect the health and metabolism of an organism. Combining nutrigenomics with longevity studies is a natural extension and promises to help identify mechanisms whereby nutrients affect the aging process, life span, and, with the incorporation of age-dependent functional measures, health span. The topics we discuss in this chapter are genetic techniques, dietary manipulations, metabolic studies, and microarray analysis methods to investigate how nutrition affects gene expression, life span, triglyceride levels, total protein levels, and live weight in Drosophila. To better illustrate nutrigenomic techniques, we analyzed Drosophila larvae or adults fed control diets (high sucrose) and compared these with larvae or adults fed diets high in the saturated fat palmitic acid, soy, or 95% lean ground beef. The main results of these studies are, surprisingly, that triglyceride and total protein levels are significantly decreased by the beef diet in all adults, and total protein levels are significantly increased in male flies fed the soy diet. Furthermore, and less surprisingly, we found that all three experimental diets significantly decreased longevity and increased the length of time to develop from egg to adult. We also describe preliminary microarray results with adult flies fed the different diets, which suggest that only about 2-3% of the approx 18,000 genes have significantly altered mRNA expression levels compared with flies fed a control sucrose diet. The significance of these results and other types of nutrigenomics and longevity analyses is discussed. |
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Authors:
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Jiatao Ye; Xiangqin Cui; Ann Loraine; Kerry Bynum; Nam Chul Kim; Gregory White; Maria De Luca; Mark D Garfinkel; Xiangyi Lu; Douglas M Ruden |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 371 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-07-19 Completed Date: 2007-09-21 Revised Date: 2007-12-03 |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 111-41 Citation Subset: IM |
Affiliation:
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Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diet* Drosophila melanogaster Enzyme Inhibitors / pharmacology Female Gene Expression Regulation / drug effects, physiology* Genes, Insect / physiology* Larva / genetics, metabolism Longevity / drug effects, physiology* Male Palmitic Acid / pharmacology RNA, Messenger / biosynthesis*, genetics Sucrose / pharmacology Sweetening Agents / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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P50DK057301/DK/NIDDK NIH HHS; R01CA105349/CA/NCI NIH HHS; R01ES92133/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/RNA, Messenger; 0/Sweetening Agents; 57-10-3/Palmitic Acid; 57-50-1/Sucrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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