| Methods for in vitro generation of human type 1 regulatory T cells. | |
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MedLine Citation:
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PMID: 20941601 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Type 1 regulatory T (Tr1) cells are adaptive regulatory T cells that are induced in the periphery upon chronic exposure to antigen (Ag) in a tolerogenic environment containing interleukin (IL)-10. Tr1 cells are Ag-specific; they produce high levels of IL-10 and TGF-β in the absence of IL-4 and suppress T-cell responses via a cytokine-dependent mechanism. During the last decade, several protocols have been developed to generate Tr1 cell lines in vitro. In this chapter, we outline protocols to generate non-Ag- and Ag-specific Tr1 cell lines and assays used to characterize Tr1 cell phenotype and functions. |
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Authors:
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Silvia Gregori; Maria Grazia Roncarolo; Rosa Bacchetta |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 677 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2011 |
Date Detail:
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Created Date: 2010-10-13 Completed Date: 2011-02-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 31-46 Citation Subset: IM |
Affiliation:
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Department of Regenerative Medicine, San Raffaele Telethon Institute for Gene Therapy HSR, Milan, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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methods Cell Proliferation* Cell Separation / methods* Cells, Cultured Cytokines / immunology* Dendritic Cells / immunology Flow Cytometry / methods Humans Interleukin-10 / immunology*, secretion Interleukin-4 / immunology Lymphocyte Activation / immunology* T-Lymphocyte Subsets / classification*, immunology T-Lymphocytes / immunology* T-Lymphocytes, Regulatory / immunology* Transforming Growth Factor beta / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Transforming Growth Factor beta; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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