Document Detail

Methods for in vitro generation of human type 1 regulatory T cells.
MedLine Citation:
PMID:  20941601     Owner:  NLM     Status:  MEDLINE    
Type 1 regulatory T (Tr1) cells are adaptive regulatory T cells that are induced in the periphery upon chronic exposure to antigen (Ag) in a tolerogenic environment containing interleukin (IL)-10. Tr1 cells are Ag-specific; they produce high levels of IL-10 and TGF-β in the absence of IL-4 and suppress T-cell responses via a cytokine-dependent mechanism. During the last decade, several protocols have been developed to generate Tr1 cell lines in vitro. In this chapter, we outline protocols to generate non-Ag- and Ag-specific Tr1 cell lines and assays used to characterize Tr1 cell phenotype and functions.
Silvia Gregori; Maria Grazia Roncarolo; Rosa Bacchetta
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  677     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-10-13     Completed Date:  2011-02-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31-46     Citation Subset:  IM    
Department of Regenerative Medicine, San Raffaele Telethon Institute for Gene Therapy HSR, Milan, Italy.
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MeSH Terms
Cell Culture Techniques / methods
Cell Proliferation*
Cell Separation / methods*
Cells, Cultured
Cytokines / immunology*
Dendritic Cells / immunology
Flow Cytometry / methods
Interleukin-10 / immunology*,  secretion
Interleukin-4 / immunology
Lymphocyte Activation / immunology*
T-Lymphocyte Subsets / classification*,  immunology
T-Lymphocytes / immunology*
T-Lymphocytes, Regulatory / immunology*
Transforming Growth Factor beta / immunology*
Reg. No./Substance:
0/Cytokines; 0/Transforming Growth Factor beta; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4

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