Document Detail

Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches.
MedLine Citation:
PMID:  20689950     Owner:  NLM     Status:  MEDLINE    
Due to the increasing demand for recombinant proteins, the interest in mammalian cell culture, especially of Chinese hamster ovary cells, grows rapidly. This is accompanied by the desire to improve cell lines in order to achieve higher titers and a better product quality. Until recently, most cell line development procedures were based on random integration and gene amplification, but several methods for targeted genetic modification of cells have been developed. Some of those are homologous recombination, RNA interference and zinc-finger nucleases. Especially the latter two have evolved considerably and will soon become a standard for cell line engineering in research and industrial application. This review presents an overview of established as well as new and promising techniques for targeted genetic modification of mammalian cells.
Oliver Krämer; Sandra Klausing; Thomas Noll
Publication Detail:
Type:  Journal Article; Review     Date:  2010-08-06
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  88     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  425-36     Citation Subset:  IM    
Bielefeld University, Germany.
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MeSH Terms
CHO Cells / metabolism*
Gene Targeting
Genetic Engineering / methods*
RNA Interference
RNA, Small Interfering
Recombinant Proteins / biosynthesis*,  genetics
Reg. No./Substance:
0/RNA, Small Interfering; 0/Recombinant Proteins

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