| Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches. | |
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MedLine Citation:
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PMID: 20689950 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Due to the increasing demand for recombinant proteins, the interest in mammalian cell culture, especially of Chinese hamster ovary cells, grows rapidly. This is accompanied by the desire to improve cell lines in order to achieve higher titers and a better product quality. Until recently, most cell line development procedures were based on random integration and gene amplification, but several methods for targeted genetic modification of cells have been developed. Some of those are homologous recombination, RNA interference and zinc-finger nucleases. Especially the latter two have evolved considerably and will soon become a standard for cell line engineering in research and industrial application. This review presents an overview of established as well as new and promising techniques for targeted genetic modification of mammalian cells. |
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Authors:
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Oliver Krämer; Sandra Klausing; Thomas Noll |
Publication Detail:
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Type: Journal Article; Review Date: 2010-08-06 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 88 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-23 Completed Date: 2011-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 425-36 Citation Subset: IM |
Affiliation:
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Bielefeld University, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals CHO Cells / metabolism* Cricetinae Cricetulus Gene Targeting Genetic Engineering / methods* Mutagenesis RNA Interference RNA, Small Interfering Recombinant Proteins / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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