Document Detail

Methods of immobilization of proteins to polymethylmethacrylate.
MedLine Citation:
PMID:  3938844     Owner:  NLM     Status:  MEDLINE    
An extracorporeal hemoperfusion chamber for insertion into the peripheral circulatory system has previously been described. In 4 to 6 hours perfusion its aim is to exert a subtractive, selective effect on the blood and to stimulate immunocompetent cells. The chamber is made from polymethylmethacrylate (PMMA). Two general methods for immobilization are described here. The first is based on hydrazinolysis of the PMMA surface. Biologically active substances can be attached through diacylhydrazine bridges, amine bonds and amide groups to the PMMA surface. In the second method carboxyl groups are generated by hydrolysis of the PMMA methyl ester groups. The biologically active substance is immobilized using diamine or dialdehyde coupling agents. Methods are provided to attach the substances to the PMMA either via their carboxyl or via their amino groups.
K Holmberg; H Hydén
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Preparative biochemistry     Volume:  15     ISSN:  0032-7484     ISO Abbreviation:  Prep. Biochem.     Publication Date:  1985  
Date Detail:
Created Date:  1986-06-02     Completed Date:  1986-06-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1276634     Medline TA:  Prep Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  309-19     Citation Subset:  IM    
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MeSH Terms
Chemical Phenomena
Hemoperfusion / instrumentation*
Staphylococcal Protein A
Reg. No./Substance:
0/Azides; 0/Borohydrides; 0/Carbodiimides; 0/Diamines; 0/Hydrazines; 0/Methylmethacrylates; 0/Proteins; 0/Staphylococcal Protein A; 111-30-8/Glutaral; 25895-60-7/sodium cyanoborohydride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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