Document Detail

Methods for functional analysis of macroautophagy in filamentous fungi.
MedLine Citation:
PMID:  19185728     Owner:  NLM     Status:  MEDLINE    
Autophagy is a bulk degradative process responsible for the turnover of membranes, organelles, and proteins in eukaryotic cells. Genetic and molecular regulation of autophagy has been independently elucidated in budding yeast and mammalian cells. In filamentous fungi, autophagy is required for several important physiological functions, such as asexual and sexual differentiation, pathogenic development, starvation stress and programmed cell death during heteroincompatibility. Here, we detail biochemical and microscopy methods useful for measuring the rate of induction of autophagy in filamentous fungi, and we summarize the methods that have been routinely used for monitoring macroautophagy in both yeast and filamentous fungi. The role of autophagy in carbohydrate catabolism and cell survival is discussed along with the specific functions of macroautophagy in fungal development and pathogenesis.
Yi Zhen Deng; Marilou Ramos-Pamplona; Naweed I Naqvi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in enzymology     Volume:  451     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2008  
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-03-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-310     Citation Subset:  IM    
Fungal Patho-Biology Group, Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore.
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MeSH Terms
Autophagy / physiology*
Biological Assay / methods*
Cadaverine / analogs & derivatives,  metabolism
Cell Membrane / metabolism,  ultrastructure
Fluorescent Dyes / metabolism
Fungi / cytology,  genetics,  metabolism*
Gene Deletion
Glycogen / metabolism
Microscopy / methods
Phagosomes / metabolism,  ultrastructure
Proteomics / methods
Reg. No./Substance:
0/Fluorescent Dyes; 10121-91-2/monodansylcadaverine; 462-94-2/Cadaverine; 9005-79-2/Glycogen

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