Document Detail

Methods for extracting biochemical information from bacterial Raman spectra: an explorative study on Cupriavidus metallidurans.
MedLine Citation:
PMID:  17386670     Owner:  NLM     Status:  MEDLINE    
In this study, we explore Raman spectra of bacteria for their biochemical information. Therefore, a database of biomolecules was used and several approaches were applied such as the study of difference spectra, the calculation dot products, the usage of coefficients obtained from an EMSC procedure and the application of 2D correlation spectroscopy. These methods were applied on a dataset containing Raman spectra of Cupriavidus metallidurans LMG 1195 in five stages of its growth, aiming to extract information about the evolution of cell components during growth. EMSC coefficients seemed to be most promising for tracking metabolic products and the results were often confirmed by difference spectra or by 2D correlation spectroscopy.
Joke De Gelder; Kris De Gussem; Peter Vandenabeele; Paul De Vos; Luc Moens
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-12
Journal Detail:
Title:  Analytica chimica acta     Volume:  585     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-27     Completed Date:  2007-07-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  234-40     Citation Subset:  IM    
Ghent University, Laboratory of Analytical Chemistry, Proeftuinstraat 86, B-9000 Ghent, Belgium.
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MeSH Terms
Acetyl Coenzyme A / chemistry
Bacteria / metabolism*
Chemistry Techniques, Analytical / methods*
Citric Acid / chemistry
Citric Acid Cycle
Cupriavidus / metabolism*
Models, Chemical
Phenylalanine / chemistry
Spectrum Analysis, Raman / methods*
Tyrosine / chemistry
Reg. No./Substance:
55520-40-6/Tyrosine; 63-91-2/Phenylalanine; 72-89-9/Acetyl Coenzyme A; 77-92-9/Citric Acid

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