| Methods developed for SELEX. | |
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MedLine Citation:
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PMID: 17072603 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification. With the development of aptamer technology over the last decade, various modified SELEX processes have arisen that allow various aptamers to be developed against a wide variety of molecules, irrespective of the target size. In the present review, the separation methods used in such SELEX processes are reviewed. |
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Authors:
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Subash Chandra Bose Gopinath |
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Publication Detail:
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Type: Journal Article; Review Date: 2006-10-28 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 387 ISSN: 1618-2642 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2007 Jan |
Date Detail:
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Created Date: 2006-12-20 Completed Date: 2007-06-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 171-82 Citation Subset: IM |
Affiliation:
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Functional Nucleic Acids Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. gopi-subashchandrabose@aist.go.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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chemistry,
isolation & purification* Chemistry Techniques, Analytical / methods SELEX Aptamer Technique / methods* |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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