| Methods for cell sorting of young and senescent cells. | |
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MedLine Citation:
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PMID: 17634572 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cellular senescence, the ultimate and irreversible loss of replicative capacity of cells in primary culture, has been a popular model for studying the aging process. However, the replicative life span of human fibroblasts is heterogeneous even in clonal populations, with the fraction of senescent cells increasing at each population doubling, rather than all cells entering senescence simultaneously. Thus, the study of individual cells in a mass culture is of extreme importance to the understanding of replicative senescence. Cell sorting is a method that allows physical separation of cells with different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations. |
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Authors:
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João F Passos; Thomas von Zglinicki |
Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 371 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-07-19 Completed Date: 2007-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 33-44 Citation Subset: IM |
Affiliation:
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Henry Wellcome Laboratory for Biogerontology Research Newcastle University, Newcastle, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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physiology* Cell Division / physiology* Cells, Cultured Fibroblasts / cytology, physiology* Flow Cytometry* / methods Humans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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