Document Detail


Methods for cell sorting of young and senescent cells.
MedLine Citation:
PMID:  17634572     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular senescence, the ultimate and irreversible loss of replicative capacity of cells in primary culture, has been a popular model for studying the aging process. However, the replicative life span of human fibroblasts is heterogeneous even in clonal populations, with the fraction of senescent cells increasing at each population doubling, rather than all cells entering senescence simultaneously. Thus, the study of individual cells in a mass culture is of extreme importance to the understanding of replicative senescence. Cell sorting is a method that allows physical separation of cells with different characteristics when measured by flow cytometry. Here, we describe various methods by which cells that reach senescence early can be physically sorted out of a bulk of growing cells, and discuss how different methods can affect the posterior analysis of the sorted populations.
Authors:
João F Passos; Thomas von Zglinicki
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  371     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-09-21     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  33-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Aging / physiology*
Cell Division / physiology*
Cells, Cultured
Fibroblasts / cytology,  physiology*
Flow Cytometry* / methods
Humans
Grant Support
ID/Acronym/Agency:
G0601333//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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