Document Detail

Methods for analysis of protein glutathionylation and their application to photosynthetic organisms.
MedLine Citation:
PMID:  19825609     Owner:  NLM     Status:  MEDLINE    
Protein S-glutathionylation, the reversible formation of a mixed-disulfide between glutathione and protein thiols, is involved in protection of protein cysteines from irreversible oxidation, but also in protein redox regulation. Recent studies have implicated S-glutathionylation as a cellular response to oxidative/nitrosative stress, likely playing an important role in signaling. Considering the potential importance of glutathionylation, a number of methods have been developed for identifying proteins undergoing glutathionylation. These methods, ranging from analysis of purified proteins in vitro to large-scale proteomic analyses in vivo, allowed identification of nearly 200 targets in mammals. By contrast, the number of known glutathionylated proteins is more limited in photosynthetic organisms, although they are severely exposed to oxidative stress. The aim of this review is to detail the methods available for identification and analysis of glutathionylated proteins in vivo and in vitro. The advantages and drawbacks of each technique will be discussed as well as their application to photosynthetic organisms. Furthermore, an overview of known glutathionylated proteins in photosynthetic organisms is provided and the physiological importance of this post-translational modification is discussed.
Xing-Huang Gao; Mariette Bedhomme; Daniel Veyel; Mirko Zaffagnini; St??phane D Lemaire
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2008-11-14
Journal Detail:
Title:  Molecular plant     Volume:  2     ISSN:  1674-2052     ISO Abbreviation:  Mol Plant     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-10-14     Completed Date:  2009-12-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465514     Medline TA:  Mol Plant     Country:  England    
Other Details:
Languages:  eng     Pagination:  218-35     Citation Subset:  IM    
Institut de Biotechnologie des Plantes, UMR 8618, CNRS/Universit?? Paris-Sud 11, B??timent 630, Orsay 91405, Cedex, France.
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MeSH Terms
Antibodies / immunology
Biotin / metabolism
Glutathione / immunology,  metabolism*
Proteins / metabolism*
Reg. No./Substance:
0/Antibodies; 0/Proteins; 58-85-5/Biotin; 70-18-8/Glutathione

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