Document Detail

Methods for the analysis of PCBs in human food, faeces and serum.
MedLine Citation:
PMID:  10481248     Owner:  NLM     Status:  MEDLINE    
A method was developed to determine trace concentrations of a range of individual PCB congeners in biological samples (serum, food and faeces) using GC-MS, to prepare a mass balance of PCBs in humans. A simple method for the analysis of PCBs in human serum, which excluded an extraction step, was first employed. Results indicated that the recoveries of 13C12 PCB spikes were variable. A soxhlet extraction step was added and was found to be efficient and reproducible. A quality control routine and method validation results are presented. In batch tests of the methods presented it was found that the serum analysis method gave within batch mean 13C12 spike recoveries of 98-120% and standard deviations between 6 and 20%. The food/faeces analysis method gave within-batch mean 13C12 spike recoveries of 88-100%, and within batch standard deviations between 4 and 12%. The batch to batch mean recovery for serum analysis was 100%, with an RSD of 9% for high spikes and 10% for low spikes. For food/faeces analysis the batch to batch average recovery was 110%, with an RSD of 5% for high spikes and 9% for low spikes.
C Y Juan; G O Thomas; K T Semple; K C Jones
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemosphere     Volume:  39     ISSN:  0045-6535     ISO Abbreviation:  Chemosphere     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-09-29     Completed Date:  1999-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320657     Medline TA:  Chemosphere     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1467-76     Citation Subset:  IM    
Institute of Environmental and Natural Science, Environmental Science Department, Lancaster University, UK.
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MeSH Terms
Carbon Isotopes
Environmental Pollutants / analysis*
Feces / chemistry
Food Contamination / analysis*
Gas Chromatography-Mass Spectrometry
Hematologic Tests / methods
Polychlorinated Biphenyls / analysis*,  blood
Sensitivity and Specificity
Reg. No./Substance:
0/Carbon Isotopes; 0/Environmental Pollutants; 0/Polychlorinated Biphenyls

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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