Document Detail

Methods for determining protein translocation by the Legionella pneumophila Dot/Icm type IV secretion system.
MedLine Citation:
PMID:  23150405     Owner:  NLM     Status:  MEDLINE    
Legionella pneumophila the causative agent of Legionnaires' disease, actively manipulates host cell -processes to establish a membrane-bound replication vacuole permissive for its replication. Establishment of such replication niche requires the Dot/Icm type IV secretion system which translocates a plethora of effectors into host cells. Determining whether a particular protein is a substrate of the transporter is a prerequisite for subsequent functional studies. Thus, a variety of methods have been developed in the last decade to measure Dot/Icm-dependent delivery of protein into the host cell. The combination of these methods and the appropriate screening strategies has allowed for the identification of more than 270 translocated proteins. These efforts have laid a solid foundation for further study of the roles of these proteins in the interactions between L. pneumophila and its host. Here, we summarized the experimental details of these methods.
Wenhan Zhu; Zhao-Qing Luo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-19     Revised Date:  2013-12-24    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  323-32     Citation Subset:  IM    
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MeSH Terms
Antigens, Bacterial / metabolism
Bacterial Proteins / metabolism*
Bacterial Secretion Systems*
Bacterial Toxins / metabolism
Cell Line
Fluorescent Antibody Technique / methods
Legionella pneumophila / metabolism*
Protein Transport
beta-Lactamases / metabolism
Grant Support
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Bacterial Toxins; 0/SidC protein, Legionella pneumophila; 0/anthrax toxin; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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