Document Detail

Methodology and evaluation of a highly sensitive algae toxicity test based on multiwell chlorophyll fluorescence imaging.
MedLine Citation:
PMID:  17118646     Owner:  NLM     Status:  MEDLINE    
A new phytotoxicity bioassay based on chlorophyll fluorescence imaging of algae suspensions in multiwell plates is introduced. Phytotoxicity is quantified via inhibition of photosystem II quantum yield, Y(II), assessed with the saturation pulse method. The basics of this approach as well as the factors enhancing and limiting its performance are outlined. Compared to other established techniques the new system allows exceptionally rapid and accurate measurements of phytotoxicity using pulse-amplitude-modulation (PAM) fluorometry. While instrument related errors are negligibly small, optimal performance depends on appropriate choice of algae and illumination conditions. Illustrative examples for the response of Phaeodactylum tricornutum to diuron are presented. The standard deviation involved in the Y(II) determination of a single well amounts to the equivalent of 44 ng/L diuron. A decisive role is played by the light (measuring light, saturation pulses, actinic light) to which samples are exposed during the bioassay: (1) the inhibitor response is enhanced at high measuring light intensity. (2) Saturation pulses may be considered non-invasive only, if applied at low frequency and as long as physiologically healthy algae cultures are used. (3) Continuous actinic light may be problematic, as it induces complex physiological reactions that limit the performance of the approach; it is not required for assessment of diuron-type inhibitors at high measuring light intensity.
Ulrich Schreiber; Pamela Quayle; Sven Schmidt; Beate I Escher; Jochen F Mueller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-21
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  22     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-06-27     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2554-63     Citation Subset:  IM    
Julius-von-Sachs Institut für Biowissenschaften, Lehrstuhl Botanik I, University of Würzburg, D-97082 Würzburg, Germany.
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MeSH Terms
Algae / cytology,  drug effects*,  metabolism*
Biological Assay / methods*
Cell Survival / drug effects
Chlorophyll / analysis*
Dose-Response Relationship, Drug
Herbicides / analysis,  toxicity*
Microscopy, Fluorescence / methods*
Reproducibility of Results
Sensitivity and Specificity
Water Pollutants, Chemical / analysis,  toxicity*
Reg. No./Substance:
0/Herbicides; 0/Water Pollutants, Chemical; 1406-65-1/Chlorophyll

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