Document Detail


Methodologies to study implantation in mice.
MedLine Citation:
PMID:  16251731     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pregnancy begins with fertilization of the ovulated oocyte by the sperm. After fertilization, the egg undergoes time-dependent mitotic division while trying to reach the blastocyst stage and the uterus for implantation. Uterine preparation for implantation is regulated by coordinated secretions and functions of ovarian sex steroids. The first sign of contact between the blastocyst and the uterus can be detected experimentally by an intravenous blue dye injection as early as the end of day 4 or the beginning of day 5 of pregnancy. This blastocyst-uterine attachment reaction leads to stromal decidual reaction only at sites of implantation. The process of implantation can be postponed and reinstated experimentally by manipulating ovarian estrogen secretion. Stromal decidualization can also be induced experimentally in the hormonally prepared uterus in response to stimuli other than the embryo. Fundamental biological questions surrounding these essential features of early pregnancy can be addressed through the application of various techniques and manipulation of this period of early pregnancy. This chapter describes the routine laboratory methodologies to study the events of early pregnancy, with special emphasis on the implantation process in mice.
Authors:
Kaushik Deb; Jeff Reese; Bibhash C Paria
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  121     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2006  
Date Detail:
Created Date:  2005-10-27     Completed Date:  2005-12-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9-34     Citation Subset:  IM    
Affiliation:
Division of Reproductive and Developmental Biology, Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA.
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MeSH Terms
Descriptor/Qualifier:
Anesthesia
Animals
Animals, Laboratory
Blastocyst
Breeding
Embryo Implantation*
Embryo Implantation, Delayed
Embryo Transfer
Estrous Cycle / physiology
Euthanasia, Animal
Female
Injections / methods
Mice
Pregnancy
Reproductive Techniques*
Time Factors
Grant Support
ID/Acronym/Agency:
HD 37394/HD/NICHD NIH HHS; HD 40193/HD/NICHD NIH HHS; HD 42636/HD/NICHD NIH HHS; HD 44741/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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