| Methodological studies on the uptake of zinc by 3T3 cells. | |
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MedLine Citation:
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PMID: 241079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the present work, experiments were conducted on the uptake of zinc by 3T3 cells. (1) The percent Zn uptake gradually decreased with addition of increasing amount of zinc (0.05-8.4 mug). (2) With the increase of the incubation period from 2 to 16 hr, Zn uptake by nearly confluent cells increases gradually; however, confluent cells which are newly replated show a distinct cyclic increase in the Zn uptake after 2, 6, and 10 hr. (3) The amino acids of DMM and serum decrease Zn uptake. (4) Histidine at a molar excess of 1:50, 1:500, and 1:5000 reduces Zn uptake in comparison to a treatment with 65Zn-Zn-L-hist2 at a molar ratio of 1:5. (5) When zinc is added in the form of different Zn compounds, at a molar ratio of 1:2 or 1:1 (Zn:ligand), EDTA decreases the Zn uptake markedly. A small influence was shown also by albumin and histidine; however, other amino and organic acids at a molar ratio of 1:2 did not alter Zn uptake significantly. |
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Authors:
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F J Schwarz; G Matrone |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) Volume: 149 ISSN: 0037-9727 ISO Abbreviation: Proc. Soc. Exp. Biol. Med. Publication Date: 1975 Sep |
Date Detail:
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Created Date: 1975-12-11 Completed Date: 1975-12-11 Revised Date: 2007-11-02 |
Medline Journal Info:
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Nlm Unique ID: 7505892 Medline TA: Proc Soc Exp Biol Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 888-92 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line* Culture Media Dose-Response Relationship, Drug Ethylenediamines / pharmacology Fumarates / pharmacology Glycine / pharmacology Histidine / pharmacology Hydrogen-Ion Concentration Mice Threonine / pharmacology Time Factors Valine / pharmacology Zinc / metabolism*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Ethylenediamines; 0/Fumarates; 56-40-6/Glycine; 7004-03-7/Valine; 71-00-1/Histidine; 72-19-5/Threonine; 7440-66-6/Zinc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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