Document Detail

Methodological evaluation of a new chemiluminescence immunoassay for thyrotropin using acridinium ester-labelled antibody.
MedLine Citation:
PMID:  2801247     Owner:  NLM     Status:  MEDLINE    
A recently available immunochemiluminometric assay (ICMA) for TSH developed by Ciba Corning Corp. has been evaluated. This system (Magic Lite) uses an acridinium-ester-labelled antibody and magnetizable particle for bound-free separation. In each assay only two calibrators are carried out and used to re-scale a manufacturer-generated curve stored in the memory of the luminometer. The precision of the response (RLU) estimated by all duplicates of 14 runs was about 4% for responses greater than 12,000 RLU (corresponding to a concentration interval 0.7-113 microIU/ml) and worsened in the lower range (up to 10% CV); the sensitivity, computed from the mean within-assay precision profile, was 0.028 microIU/ml; the between-assay precision ranged from 4.6 to 13.1 CV%. Regression analysis of ICMA results (y) against consensus values of Behring IRMA (x) on 15 QC sera assayed in an inter-laboratory survey (concentration range 1-30 microIU/ml) gave y = -0.003 + 0.98x indicating a good agreement of the two techniques. Similar conclusions have been derived from the comparison of the ICMA results (y) in the low TSH concentration range (less than 1 microIU/ml) against the IRMA Boots Celltech (x) on 80 patient samples (y = 0.04 + 1.04x).
G C Zucchelli; A Pilo; S Masini; M R Chiesa; A Masi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of bioluminescence and chemiluminescence     Volume:  4     ISSN:  0884-3996     ISO Abbreviation:  J. Biolumin. Chemilumin.     Publication Date:  1989 Jul 
Date Detail:
Created Date:  1989-11-06     Completed Date:  1989-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8612490     Medline TA:  J Biolumin Chemilumin     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  620-6     Citation Subset:  IM    
C. N. R. Institute of Clinical Physiology, Pisa, Italy.
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MeSH Terms
Immunoassay / methods*
Luminescent Measurements*
Quality Control
Reference Standards
Thyrotropin / blood*,  standards
Reg. No./Substance:
0/Acridines; 0/Esters; 9002-71-5/Thyrotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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