| Methodological assessment of acid-etching for visualizing the osteocyte lacunar-canalicular networks using scanning electron microscopy. | |
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MedLine Citation:
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PMID: 19725069 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Osteocytes are the most abundant of the bone cells. Each osteocyte is contained within its own lacuna and connected to adjacent osteocytes via fillipodial processes, which form an intricate network of canaliculi within the matrix. Studying this intricate network of cells and their processes is difficult, because it exists embedded within a densely mineralized matrix. Scanning electron microscopy (SEM) has been shown to be a useful tool for visualizing this cellular network, yet the techniques involved for preparing specimens has not been systematically explored. The goal of this study was to investigate how variations in acid-etching, both etching media and etching duration, affect SEM-based visualization of the osteocyte lacunar-canalicular network. Bone samples were embedded in plastic and then acid etched in either 9% (10, 20, 40, and 60 s durations) or 37% (5, 10, and 15 s) phosphoric acid. Specimens were imaged using SEM, and qualitative evaluation of the lacunar-canalicular network was undertaken. Our findings show acid etchingwith a 9% phosphoric acid solution for 20 s provided the most favorable visualization of the osteocyte lacunar-canalicular network. |
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Authors:
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Daniel J Kubek; Vincent H Gattone; Matthew R Allen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microscopy research and technique Volume: 73 ISSN: 1097-0029 ISO Abbreviation: Microsc. Res. Tech. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9203012 Medline TA: Microsc Res Tech Country: United States |
Other Details:
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Languages: eng Pagination: 182-6 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone and Bones / cytology*, ultrastructure* Dental Materials / pharmacology Dogs Female Histological Techniques / methods* Microscopy, Electron, Scanning Osteocytes / ultrastructure* Phosphoric Acids / pharmacology Specimen Handling / methods* |
| Chemical | |
Reg. No./Substance:
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0/Dental Materials; 0/Phosphoric Acids; 7664-38-2/phosphoric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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