Document Detail


Method for visualizing aerosol contamination in flow sorters.
MedLine Citation:
PMID:  18770766     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The routine operation of a flow cytometer generates aerosols, and most commercially available instrument are not designed to be 100% effective in their elimination. Aerosols are a concern for operators as well as for others in the facility because of the potential for exposure to contamination, especially during sorting of biohazardous samples. This unit describes a visual method for examining aerosol containment using a commercial resin that fluoresces under UV or black light illumination. The method is rapid, inexpensive, and simple. Results are immediate and potentially quantitative.
Authors:
Andrew S Oberyszyn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]     Volume:  Chapter 3     ISSN:  1934-9300     ISO Abbreviation:  Curr Protoc Cytom     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2008-09-04     Completed Date:  2008-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100899351     Medline TA:  Curr Protoc Cytom     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 3.5     Citation Subset:  IM    
Affiliation:
The Ohio State University Comprehensive Cancer Center, Analytical Cytometry Shared Resource Laboratory, Ohio State University, Columbus, Ohio, USA.
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MeSH Terms
Descriptor/Qualifier:
Aerosols*
Cell Separation / instrumentation,  methods
Equipment Design / methods
Flow Cytometry / instrumentation*,  methods
Hazardous Substances / analysis
Light
Reproducibility of Results
Safety
Ultraviolet Rays
Chemical
Reg. No./Substance:
0/Aerosols; 0/Hazardous Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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