| A method to inhibit the growth of Plasmodium falciparum by double-stranded RNA-mediated gene silencing of helicases. | |
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MedLine Citation:
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PMID: 20225164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Malaria in human is caused by four Plasmodium species, with Plasmodium falciparum responsible for the most severe form of the disease. Global resistance to multiple antimalarial drugs is becoming a major challenge in worldwide efforts to control malaria. It is essential to identify new targets. One possible target is helicases, which are important ubiquitous unwinding enzymes required for nucleic acid metabolism and the maintenance of genomic stability. Helicases are motor proteins that use the energy derived from their intrinsic nucleic acid-dependent NTPase activity to unwind the duplex nucleic acid substrate. In this chapter, we study the functional role of helicases in malaria parasite by using specific dsRNA against PfH45, one of the parasite helicases. We describe the methods for Plasmodium falciparum culture, the amplification of specific helicase gene, the construction of specific dsRNA, and the analysis of the effect of dsRNA on parasite growth. Using this approach, we show that helicases are indispensable enzymes, which are required for growth and most probably survival of the malaria parasite. |
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Authors:
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Renu Tuteja |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 587 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-03-12 Completed Date: 2010-05-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 389-99 Citation Subset: IM |
Affiliation:
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Malaria Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals DNA Helicases* / antagonists & inhibitors, genetics, metabolism Gene Silencing* Humans Plasmodium falciparum* / enzymology, genetics, growth & development RNA, Double-Stranded / genetics, metabolism* Recombinant Fusion Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Double-Stranded; 0/Recombinant Fusion Proteins; EC 3.6.1.-/DNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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