| Method for the simultaneous determination of retinol and beta-carotene concentrations in human tissues and plasma. | |
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MedLine Citation:
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PMID: 11969193 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To understand differential tissue distribution of retinoids and carotenoids, as it might influence biological processes in humans, we developed and demonstrated a method for measuring them in selected human tissues. The method includes internal standards and a secondary reference standard to eliminate the need for external standard calibration and to minimize sample-handling errors. Tissues were digested (saponified) in ethanolic KOH. Retinol and beta-carotene were extracted with organic solvent containing internal standards. Analytes were separated using isocratic liquid chromatography and quantified at 325 nm for retinol and 450 nm for beta-carotene. Plasma was analyzed in a similar way but without saponification. Retinal-O-ethyloxime and beta-apo-12'-carotenal-O-t-butyloxime served as internal standards. Plasma, breast, and fat from breast surgery patients and colon, liver, muscle, and fat from colon surgery patients were analyzed. Within-day relative standard deviations (RSDs) for plasma were <0.04 for beta-carotene and <0.03 for retinol, between-day RSDs were <0.05 for beta-carotene and <0.04 for retinol. Saponification ensured complete extraction of retinol and beta-carotene and removal of triglycerides that "foul" chromatographic columns. It seems retinol and beta-carotene concentrations in tissues and blood of cancer patients are the same or higher than those in corresponding tissues of patients without these cancers. |
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Authors:
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Jennine M Lunetta; Rebecca A Zulim; Stephen R Dueker; Yumei Lin; Vicky Flaig; Philip D Schneider; Bruce M Wolfe; Andrew J Clifford |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 304 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-04-23 Completed Date: 2002-10-02 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 100-9 Citation Subset: IM |
Copyright Information:
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(c)2002 Elsevier Science (USA). |
Affiliation:
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Department of Nutrition, University of California at Davis, 95616, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Blood Chemical Analysis / methods Breast Neoplasms / blood, chemistry Chromatography, High Pressure Liquid Colonic Neoplasms / blood, chemistry Female Humans Male Middle Aged Reference Standards Spectrophotometry Tissue Distribution Vitamin A / analysis*, blood*, standards beta Carotene / analysis*, blood*, standards |
| Grant Support | |
ID/Acronym/Agency:
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DK-43098/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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11103-57-4/Vitamin A; 7235-40-7/beta Carotene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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