Document Detail


Method for the simultaneous determination of retinol and beta-carotene concentrations in human tissues and plasma.
MedLine Citation:
PMID:  11969193     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To understand differential tissue distribution of retinoids and carotenoids, as it might influence biological processes in humans, we developed and demonstrated a method for measuring them in selected human tissues. The method includes internal standards and a secondary reference standard to eliminate the need for external standard calibration and to minimize sample-handling errors. Tissues were digested (saponified) in ethanolic KOH. Retinol and beta-carotene were extracted with organic solvent containing internal standards. Analytes were separated using isocratic liquid chromatography and quantified at 325 nm for retinol and 450 nm for beta-carotene. Plasma was analyzed in a similar way but without saponification. Retinal-O-ethyloxime and beta-apo-12'-carotenal-O-t-butyloxime served as internal standards. Plasma, breast, and fat from breast surgery patients and colon, liver, muscle, and fat from colon surgery patients were analyzed. Within-day relative standard deviations (RSDs) for plasma were <0.04 for beta-carotene and <0.03 for retinol, between-day RSDs were <0.05 for beta-carotene and <0.04 for retinol. Saponification ensured complete extraction of retinol and beta-carotene and removal of triglycerides that "foul" chromatographic columns. It seems retinol and beta-carotene concentrations in tissues and blood of cancer patients are the same or higher than those in corresponding tissues of patients without these cancers.
Authors:
Jennine M Lunetta; Rebecca A Zulim; Stephen R Dueker; Yumei Lin; Vicky Flaig; Philip D Schneider; Bruce M Wolfe; Andrew J Clifford
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  304     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-04-23     Completed Date:  2002-10-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  100-9     Citation Subset:  IM    
Copyright Information:
(c)2002 Elsevier Science (USA).
Affiliation:
Department of Nutrition, University of California at Davis, 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Blood Chemical Analysis / methods
Breast Neoplasms / blood,  chemistry
Chromatography, High Pressure Liquid
Colonic Neoplasms / blood,  chemistry
Female
Humans
Male
Middle Aged
Reference Standards
Spectrophotometry
Tissue Distribution
Vitamin A / analysis*,  blood*,  standards
beta Carotene / analysis*,  blood*,  standards
Grant Support
ID/Acronym/Agency:
DK-43098/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
11103-57-4/Vitamin A; 7235-40-7/beta Carotene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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