Document Detail

Method for the sequential online analysis of enzyme reactions based on capillary electrophoresis.
MedLine Citation:
PMID:  22432866     Owner:  NLM     Status:  In-Data-Review    
We have developed an easy-to-operate and effective method for performing the sequential online analysis of enzyme reactions based on capillary electrophoresis (CE). The system was constructed by passing two capillaries through a sample vial at a distance of 5 μm between the capillary ends. Direct online sample injection and sequential CE analysis were achieved by periodically switching the high-voltage power supply off and on, without any physical disturbance of the capillary inlet. The sample was injected via concentration diffusion with in-column derivatization of the amino acids occurring at the interface of the capillaries. High reproducibility of the sequential injections was demonstrated with relative standard deviation values (n = 20) of 1.01%, 1.25%, and 0.80% for peak height, peak area, and migration time, respectively. Sequential online CE enzyme assay of a glutamate pyruvate transaminase catalyzed enzyme reaction was carried out by simultaneously monitoring the substrate consumption and the product formation every 30 s from the beginning to the end of the reaction. The Michaelis constants for the reaction were obtained and were found to be in good agreement with the results of traditional off-line enzyme assays. Our method has great potential for usage in sequential online CE analysis of chemical reactions with in-column chemical derivatization of the analytes for ultraviolet or laser-induced fluorescence detection.
Yuanfang Chen; Liangliang Xu; Wenwen Zhao; Liping Guo; Li Yang
Related Documents :
21315896 - Application of cement as new electrode material and solid-phase microextraction materia...
16024936 - Presence of surfactant lamellar bodies in normal and diseased sinus mucosa.
21941916 - Antioxidant capacity and larvicidal and antifungal activities of essential oils and ext...
11380516 - Multiple inert gas elimination technique for determining ventilation/perfusion distribu...
19212046 - High-precision detection method of emission signals from a pulsed radio-frequency glow ...
23390086 - Cyclic sample pooling using two-dimensional liquid chromatography system enhances cover...
Publication Detail:
Type:  Journal Article     Date:  2012-03-08
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2961-7     Citation Subset:  IM    
Faculty of Chemistry, Northeast Normal University , Changchun, Jilin, 130024, P.R.China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Exercise training utilizing body weight-supported treadmill walking with a young adult with cerebral...
Next Document:  Ethics in Electrophysiology: Who Speaks for this Man?