Document Detail


Method for selecting anthocyanin-producing cells by a cell sorter.
MedLine Citation:
PMID:  8073093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We found a novel method to select anthocyanin-producing cells by a new means of cell staining. After staining with 0.2 ppm fluorescein isothiocyanate (FITC), the protoplasts from Aralia cordata cultured cells, which were composed of high anthocyanin-producing cells and non-producing cells, were observed to be distinguishable under a fluorescence microscope. The green fluorescence of FITC from the non-producing cells was clearly ascertained by the naked eye. On the other hand, the fluorescence of the anthocyanin-producing cells was completely missing. This phenomenon resulted from compensation of the green fluorescence (lambda max 525 nm) of FITC and the green light absorption (lambda max 530 nm) of anthocyanin. Although investigations of the flow cytometric method were developed on the basis of this phenomenon, the histograms of these protoplasts expressed the distinction between the anthocyanin-producing cells and the non-producing cells. In order to prove the technique, a highly productive cell line was selected from the heterogeneous populations of anthocyanin-producing or non-producing protoplasts from A. cordata cultured cells. The number of the sorted protoplasts containing anthocyanin was counted by the naked eye, the anthocyanin-producing protoplasts were approximately 90% in the whole protoplasts and the viability was 60-70%. Anthocyanin concentration the sorted protoplasts was 3-fold higher as compared with the protoplasts before sorting. The anthocyanin content of the cell line selected by this method was 7.7 +/- 0.6%/g dw.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
K Sakamoto; K Iida; T Koyano; Y Asada; T Furuya
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta medica     Volume:  60     ISSN:  0032-0943     ISO Abbreviation:  Planta Med.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2002-12-13    
Medline Journal Info:
Nlm Unique ID:  0066751     Medline TA:  Planta Med     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  253-9     Citation Subset:  IM    
Affiliation:
Saitama Laboratory, P.C.C. Technology Inc., Japan.
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MeSH Terms
Descriptor/Qualifier:
Anthocyanins / biosynthesis*
Cell Line
Cell Separation / instrumentation*
Flow Cytometry
Plants / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Anthocyanins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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