| Method for selecting anthocyanin-producing cells by a cell sorter. | |
| | |
MedLine Citation:
|
PMID: 8073093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We found a novel method to select anthocyanin-producing cells by a new means of cell staining. After staining with 0.2 ppm fluorescein isothiocyanate (FITC), the protoplasts from Aralia cordata cultured cells, which were composed of high anthocyanin-producing cells and non-producing cells, were observed to be distinguishable under a fluorescence microscope. The green fluorescence of FITC from the non-producing cells was clearly ascertained by the naked eye. On the other hand, the fluorescence of the anthocyanin-producing cells was completely missing. This phenomenon resulted from compensation of the green fluorescence (lambda max 525 nm) of FITC and the green light absorption (lambda max 530 nm) of anthocyanin. Although investigations of the flow cytometric method were developed on the basis of this phenomenon, the histograms of these protoplasts expressed the distinction between the anthocyanin-producing cells and the non-producing cells. In order to prove the technique, a highly productive cell line was selected from the heterogeneous populations of anthocyanin-producing or non-producing protoplasts from A. cordata cultured cells. The number of the sorted protoplasts containing anthocyanin was counted by the naked eye, the anthocyanin-producing protoplasts were approximately 90% in the whole protoplasts and the viability was 60-70%. Anthocyanin concentration the sorted protoplasts was 3-fold higher as compared with the protoplasts before sorting. The anthocyanin content of the cell line selected by this method was 7.7 +/- 0.6%/g dw.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
K Sakamoto; K Iida; T Koyano; Y Asada; T Furuya |
Related Documents
:
|
18493083 - A new purple fluorescent color marker for genetic studies in saccharomyces cerevisiae a... 8699033 - Time-resolved fluorometric assay for natural killer activity using target cells labelle... 10684443 - Electrophysiology of myocardial cells in the epicardial, midmyocardial, and endocardial... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Planta medica Volume: 60 ISSN: 0032-0943 ISO Abbreviation: Planta Med. Publication Date: 1994 Jun |
Date Detail:
|
Created Date: 1994-09-29 Completed Date: 1994-09-29 Revised Date: 2002-12-13 |
Medline Journal Info:
|
Nlm Unique ID: 0066751 Medline TA: Planta Med Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 253-9 Citation Subset: IM |
Affiliation:
|
Saitama Laboratory, P.C.C. Technology Inc., Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anthocyanins
/
biosynthesis* Cell Line Cell Separation / instrumentation* Flow Cytometry Plants / cytology, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Anthocyanins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypoglycemic and antihyperglycemic effects of Zizyphus spina-christi in rats.
Next Document: The relationships between RBE and LET for different types of lethal damage in mammalian cells: bioph...