| Method for resolution and quantification of components of the non-photochemical quenching (q (N)). | |
| | |
MedLine Citation:
|
PMID: 20535559 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
A new method of the chlorophyll (Chl) a fluorescence quenching analysis is described, which allows the calculation of values of (at least) three components of the non-photochemical quenching of the variable Chl a fluorescence (q (N)) using a non-linear regression of a multi-exponential function within experimental data. Formulae for coefficients of the "energy"-dependent (DeltapH-dependent) quenching (q (E)), the state-transition quenching (q (T)) and the photo/inhibitory quenching (q (I)) of Chl a fluorescence were found on the basis of three assumptions: (i) the dark relaxation kinetics of q (N), as well as of all its components, is of an exponential nature, (ii) the superposition principle is valid for individual Chl a fluorescence quenching processes and (iii) the same reference fluorescence level (namely the maximum variable Chl a fluorescence yield in the dark-adapted state, F (V)) is used to define both q (N) and its components. All definitions as well as the algorithms for analytical recognition of the q (N) components are theoretically clarified and experimentally tested. The described theory results in a rather simple equation allowing to compute values for all q (N) components (q (E), q (T), q (I)) as well as the half-times of relaxation (tau(1/2)) of corresponding quenching processes. It is demonstrated that under the above assumptions it holds: q (N) = q (E) + q (T) + q (I). The theoretically derived equations are tested, and the results obtained are discussed for non-stressed and stressed photosynthetically active samples. Semi-empirical formulae for a fast estimation of values of the q (N) components from experimental data are also given. |
| | |
Authors:
|
Karel Rohácek |
Related Documents
:
|
15607089 - A method for comparing group fmri data using independent component analysis: applicatio... 18222529 - Application of factorial kriging analysis to the foregs european topsoil geochemistry d... 19671149 - A structured overview of simultaneous component based data integration. 20556849 - Principal manifolds and graphs in practice: from molecular biology to dynamical systems. 17015309 - Environmental forcing as a main determinant of bloom dynamics of the chrysochromulina a... 1938139 - Determination of the number and waveshapes of event related potential components using ... 23204999 - Lost in translation? the dilemma of alignment within participatory technology developme... 23381499 - On the optimal size of marine reserves. 1838519 - Experimental and analytical techniques used in bird orientation research. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-11 |
Journal Detail:
|
Title: Photosynthesis research Volume: 105 ISSN: 1573-5079 ISO Abbreviation: Photosyn. Res. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100954728 Medline TA: Photosynth Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 101-13 Citation Subset: IM |
Affiliation:
|
Biology Centre of the Academy of Sciences of the Czech Republic, p.r.i., Institute of Plant Molecular Biology, Branisovská 31, 370 05 Ceske Budejovice, Czech Republic. rohacek@umbr.cas.cz |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of microvessels in colorectal tumors by narrow band imaging magnification: including comp...
Next Document: Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthes...