Document Detail


Method for quantitative detection and presumptive identification of group B streptococci on primary plating.
MedLine Citation:
PMID:  12682120     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recommended by the Centers for Disease Control and Prevention. This technique is sensitive; it does not, however, allow either evaluation of the degree of colonization or detection of cocolonization with several GBS clones. We have examined the carriage rate and population dynamics of GBS in a group of Danish women during pregnancy and 1 year after delivery using a new detection method. In the present paper we describe a mixed blood agar medium (MB agar) that identifies GBS in the primary cultures by detection of a double hemolysis pattern consisting of characteristic, large zones of partial hemolysis ("CAMP zones") and of narrow zones of complete hemolysis. The MB agar was at least as sensitive as culturing in selective broth for detection of GBS in vaginal and anorectal swab specimens, and GBS strains could be identified directly on the primary plate due to the CAMP zones without the need for subculturing. The carriage rate of GBS in a group of Danish women was found to be more than 30%, a figure considerably higher than the rate that was reported previously.
Authors:
Søren Mose Hansen; Uffe B Skov Sørensen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  41     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-08     Completed Date:  2003-07-11     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1399-403     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Agar
Bacterial Proteins / metabolism
Bacterial Typing Techniques*
Blood
Carrier State / microbiology*
Culture Media
Female
Hemolysin Proteins
Hemolysis
Humans
Pregnancy
Pregnancy Complications, Infectious / diagnosis*,  microbiology
Rectum / microbiology
Streptococcal Infections / diagnosis*,  microbiology
Streptococcus agalactiae / classification,  growth & development*,  isolation & purification*
Vagina / microbiology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/CAMP protein, Streptococcus; 0/Culture Media; 0/Hemolysin Proteins; 9002-18-0/Agar
Comments/Corrections

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