Document Detail


Method for improved extraction of DNA from Nocardia asteroides.
MedLine Citation:
PMID:  2671036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.
Authors:
M J Loeffelholz; D R Scholl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  27     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-10-12     Completed Date:  1989-10-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1880-1     Citation Subset:  IM    
Affiliation:
Diagnostic Hybrids, Inc., Athens, Ohio 45701.
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MeSH Terms
Descriptor/Qualifier:
DNA Probes
DNA, Bacterial / analysis,  isolation & purification*
Electrophoresis, Agar Gel
Nocardia asteroides / genetics*
Sodium Dodecyl Sulfate
Chemical
Reg. No./Substance:
0/DNA Probes; 0/DNA, Bacterial; 151-21-3/Sodium Dodecyl Sulfate
Comments/Corrections

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