| Method for evaluating the potential of C labeled plant polyphenols to cross the blood-brain barrier using accelerator mass spectrometry. | |
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MedLine Citation:
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PMID: 20419067 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Bioactive compounds in botanicals may be beneficial in preventing age-related neurodegenerative diseases, but for many compounds conventional methods may be inadequate to detect if these compounds cross the blood brain barrier or to track the pharmacokinetics in the brain. By combining a number of unique technologies it has been possible to utilize the power of AMS to study the pharmacokinetics of bioactive compounds in the brain at very low concentrations. (14)C-labeled compounds can be biosynthesized by plant cell suspension cultures co-incubated with radioisotopically-labeled sucrose and isolated and separated into a series of bioactive fractions.To study the pharmacokinetics and tissue distribution of (14)C labeled plant polyphenols, rats were implanted with jugular catheters, subcutaneous ultrafiltration probes and brain microdialysis probes. Labeled fractions were dosed orally. Interstitial fluid (ISF) and brain microdialysate samples were taken in tandem with blood samples. It was often possible to determine (14)C in blood and ISF with a beta-counter. However, brain microdialysate samples (14)C levels on the order of 10(7) atoms/sample required AMS technology. The Brain Microdialysate(AUC)/Serum(AUC) ranged from .021- to .029, with the higher values for the glycoside fractions. By using AMS in combination with traditional methods, it is possible to study uptake by blood, distribution to ISF and determine the amount of a dose which can reach the brain and follow the pharmacokinetics in the brain. |
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Authors:
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Elsa M Janle; Mary Ann Lila; Michael Grannan; Lauren Wood; Aine Higgins; Gad G Yousef; Randy B Rogers; Helen Kim; George S Jackson; Connie M Weaver |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms Volume: 268 ISSN: 0168-583X ISO Abbreviation: - Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9881766 Medline TA: Nucl Instrum Methods Phys Res B Country: - |
Other Details:
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Languages: ENG Pagination: 1313-1316 Citation Subset: - |
Affiliation:
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Purdue University, Department of Foods and Nutrition, West Lafayette, IN, USA. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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P50 AT000477-01//NCCAM NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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