Document Detail


Method for determining molar concentrations of metabolites in complex solutions from two-dimensional 1H-13C NMR spectra.
MedLine Citation:
PMID:  17985927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One-dimensional (1D) (1)H nuclear magnetic resonance (NMR) spectroscopy is used extensively for high-throughput analysis of metabolites in biological fluids and tissue extracts. Typically, such spectra are treated as multivariate statistical objects rather than as collections of quantifiable metabolites. We report here a two-dimensional (2D) (1)H-(13)C NMR strategy (fast metabolite quantification, FMQ, by NMR) for identifying and quantifying the approximately 40 most abundant metabolites in biological samples. To validate this technique, we prepared mixtures of synthetic compounds and extracts from Arabidopsis thaliana, Saccharomyces cerevisiae, and Medicago sativa. We show that accurate (technical error 2.7%) molar concentrations can be determined in 12 min using our quantitative 2D (1)H-(13)C NMR strategy. In contrast, traditional 1D (1)H NMR analysis resulted in 16.2% technical error under nearly ideal conditions. We propose FMQ by NMR as a practical alternative to 1D (1)H NMR for metabolomics studies in which 50-mg (extract dry weight) samples can be obtained.
Authors:
Ian A Lewis; Seth C Schommer; Brendan Hodis; Kate A Robb; Marco Tonelli; William M Westler; Michael R Sussman; John L Markley
Related Documents :
23680387 - In situ derivatization of metals and organometallics using borate reagents in gas chrom...
24751297 - Estrogen degradation and sorption onto colloids in a constructed wetland with different...
19937967 - Direct purification of tanshinones from salvia miltiorrhiza bunge by high-speed counter...
22971167 - Capillary electrophoresis mass spectrometry for the characterization of o-acetylated n-...
13929797 - Chromatographic resolution of the first component of human complement into three activi...
7795387 - N-glycosylation site mapping of recombinant tissue plasminogen activator by micellar el...
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2007-11-07
Journal Detail:
Title:  Analytical chemistry     Volume:  79     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-14     Completed Date:  2008-02-21     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9385-90     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Arabidopsis
Body Fluids / chemistry*
Carbon Isotopes
Magnetic Resonance Spectroscopy / methods*
Medicago sativa
Metabolism*
Methods
Reproducibility of Results
Saccharomyces cerevisiae
Solutions
Grant Support
ID/Acronym/Agency:
1T32HG002760/HG/NHGRI NIH HHS; P41 GM GM66326/GM/NIGMS NIH HHS; P41 GM066326/GM/NIGMS NIH HHS; P41 GM066326-05/GM/NIGMS NIH HHS; P41 RR002301/RR/NCRR NIH HHS; P41 RR002301-15/RR/NCRR NIH HHS; P41 RR02301/RR/NCRR NIH HHS; R21 DK070297/DK/NIDDK NIH HHS; R21 DK070297-01/DK/NIDDK NIH HHS; T32 HG002760/HG/NHGRI NIH HHS; T32 HG002760-05/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Solutions
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Target and nontarget screening of organic micropollutants in water by solid-phase microextraction co...
Next Document:  Characterization of nonenzymatic glycation on a monoclonal antibody.