Document Detail

Method of determination of appropriate heat treatment of animal meal by immunoassay developed for detection of cooked beef: interlaboratory study.
MedLine Citation:
PMID:  11767153     Owner:  NLM     Status:  MEDLINE    
An interlaboratory trial was conducted for the validation of an enzyme-linked immunosorbent assay (ELISA) method for determination of appropriate heat treatment of animal meal. A commercially available ELISA test kit developed for the identification of beef in cooked food was used in the study. Twelve laboratories from 7 European countries examined 2 different analytical protocols to establish the most appropriate analytical method. Three different samples were used, 2 animal waste materials sterilized at 129 and 134 degrees C (wet conditions), respectively, and a meat and bone meal material processed at dry conditions (maximum temperature, 140 degrees C). Statistical evaluation applying t-statistics showed that the animal meal treated according to European legislation (>133 degrees C) was clearly distinguishable from the 2 other test materials at a 99% confidence level using both analytical protocols. This method can be considered as a complementary test to the immunoassay developed for the detection of pork in cooked food that is already applied in routine analysis for the surveillance of rendering plants.
E Björklund; L Pallaroni; C von Holst; W Unglaub
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  84     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2001-12-19     Completed Date:  2002-06-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1839-45     Citation Subset:  IM    
European Commission, Joint Research Centre, Food Products and Consumer Goods Unit, Ispra (VA), Italy.
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MeSH Terms
Animal Feed / analysis*
Enzyme-Linked Immunosorbent Assay / methods*,  statistics & numerical data
European Union
Food Contamination / prevention & control
Hot Temperature
Meat / analysis*

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