Document Detail

Method for autologous single skin cell isolation for regenerative cell spray transplantation with non-cultured cells.
MedLine Citation:
PMID:  21480179     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: There is a therapeutic gap for patients with deep partial thickness wounds (Grade IIb) of moderate size that were initially not treated with split- or mesh grafting to avoid overgrafting, but developed delayed wound healing around two weeks after injury--at which time grafting is typically not indicated anymore. Delayed wound healing is often associated with esthetically unsatisfactory results and sometimes functional problems. An innovative cell isolation method for cell spray transplantation at the point of care, which eliminates cell culture prior to treatment, was implemented for this population of burn patients in our center.
METHODS: Autologous skin cell spray transplantation was initiated by taking healthy skin. The dermal/epidermal layers were separated using enzymatic digestion with 40 min dispase application, followed by 15 min trypsin application for basal kerationcyte isolation, 7 min cell washing by centrifugation, followed by transferring the cells for spraying into Ringer lactate solution. The procedure was performed on site in a single session immediately following the biopsy. After sharp wound debridement, cells were immediately transplanted by deposition with a cell sprayer for even distribution of the cell suspension.
RESULTS AND CONCLUSIONS: Eight patients were treated (mean age 30.3 years, mean burn total body surface area 14%, mean Abbreviated Burn Severity Index (5 points). The mean time to complete re-epithelialization was 12.6 days. All patients exhibited wound healing with improved esthetic and functional quality. Our initial experience for the use of non-cultured cells using a two-enzyme approach with cell washing suggests shortened time for wound closure, suggesting that the method may potentially avoid longer-term complications.
Jörg C Gerlach; Christa Johnen; Christian Ottomann; Christian Ottoman; Kirsten Bräutigam; Jörn Plettig; Claudia Belfekroun; Sandra Münch; Bernd Hartmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of artificial organs     Volume:  34     ISSN:  1724-6040     ISO Abbreviation:  Int J Artif Organs     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-04-11     Completed Date:  2011-08-08     Revised Date:  2014-07-22    
Medline Journal Info:
Nlm Unique ID:  7802649     Medline TA:  Int J Artif Organs     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  271-9     Citation Subset:  IM    
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MeSH Terms
Burns / surgery*
Cell Transplantation / methods*
Child, Preschool
Middle Aged
Skin / cytology*
Skin Transplantation / methods*
Treatment Outcome
Wound Healing / physiology*
Erratum In:
Int J Artif Organs. 2014 Feb;37(2):184
Note: Ottoman, Christian [corrected to Ottomann, Christian]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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