Document Detail


Method for the accurate preparation of cell-spiking standards.
MedLine Citation:
PMID:  19115959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Preparation of calibration standards for cell enumeration is critical in characterizing the performance of any method or apparatus intended for recovering rare cells. Diluting a cell suspension serially is prone to statistical sampling errors as the cell suspension becomes more dilute, whereas transferring and injecting cells individually into a diluent with a micromanipulator is time-consuming. We developed a simple and robust method using a surface-modified glass capillary to siphon and eject cells. One-dimensional confinement of cells offered by the capillary made cell enumeration by visual counting simple and rapid, and cell ejection from the capillary was near 100% when the appropriate surface coating and cell solution was used. The residence time of cells in the capillary, however, could affect the percentage of cells that was ejected from the capillary. To characterize the performance of this method, we enumerated the ejected cell using both visual counting under a microscope and automated detection using a chip-based flow cytometer.
Authors:
Yongxi Zhao; Perry G Schiro; Jason S Kuo; Laiying Ng; Daniel T Chiu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  81     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-30     Completed Date:  2009-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1285-90     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Count / standards*
Cell Line, Tumor
Cell Survival
Flow Cytometry / methods*
Glass / chemistry
Humans
Reference Standards
Silanes / chemistry
Staining and Labeling
Surface Properties
Chemical
Reg. No./Substance:
0/Silanes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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