| Method for umbilical cord blood-derived basophils by FCM. | |
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MedLine Citation:
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PMID: 20715997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have devised a method for the purification of human basophils from umbilical cord blood by FCM. Umbilical cord blood was collected from six healthy, full-term deliveries. After separation of red blood cells, mononuclear cells were isolated by Ficoll-Hypaque. Six samples followed by positive selection using flowcytometry (FCM) for CD203c(+)CD45(int+) cells. Purity and recovery of cells were measured. Purity and recovery of basophils by FCM with CD203c(+)CD45(int+) markers were 95.02 +/- 2.94% and 61.42 +/- 5.95%. Cell sorting for CD203c(+)CD45(int+) cells by FCM is an improved method for obtaining pure umbilical cord blood-derived basophils. We established cord blood-derived basophil purification technique as a source from which active basophils can be isolated for immunochemical characterization. |
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Authors:
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Ling Yang; Wei-Guo Xu; Yi-Ping Xu; Su-Hang Yao; Yin-Shi Guo; Ke-Min Wang; Ying Xiong; Jie Zhu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Hybridoma (2005) Volume: 29 ISSN: 1557-8348 ISO Abbreviation: Hybridoma (Larchmt) Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-18 Completed Date: 2010-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101241539 Medline TA: Hybridoma (Larchmt) Country: United States |
Other Details:
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Languages: eng Pagination: 367-70 Citation Subset: IM |
Affiliation:
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Respiratory Department, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD45
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immunology,
metabolism Basophils / cytology*, physiology Cell Separation Fetal Blood / cytology* Ficoll / chemistry Flow Cytometry* Humans Leukocytes / cytology, immunology Phosphoric Diester Hydrolases / immunology, metabolism Pyrophosphatases / immunology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/ENPP3 protein, human; 25702-74-3/Ficoll; EC 3.1.3.48/Antigens, CD45; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.6.1.-/Pyrophosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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