| A Method for Enhancing Cell-penetration of Gd<sup>3+</sup>-based MRI Contrast Agents by Conjugation with Hydrophobic Fluorescent Dyes. | |
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MedLine Citation:
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PMID: 21985057 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Gadolinium ion (Gd<sup>3+</sup>) complexes are commonly used as magnetic resonance imaging (MRI) contrast agents to enhance signals in <i>T</i><sub>1</sub>-weighted MR images. Recently, several methods to achieve cell-permeation of Gd<sup>3+</sup> complexes have been reported, but more general and efficient methodology is needed. In this report, we describe a novel method to achieve cell-permeation of Gd<sup>3+</sup> complexes by using hydrophobic fluorescent dyes as a cell-permeability-enhancing unit. We synthesized Gd<sup>3+</sup> complexes conjugated with boron dipyrromethene (<b>BDP-Gd</b>) and Cy7 dye (<b>Cy7-Gd</b>), and showed that these conjugates can be introduced efficiently into cells. To examine the relationship between cell-permeability and dye structure, we further synthesized a series of <b>Cy7-Gd</b> derivatives. On the basis of MR imaging, flow cytometry and ICP-MS analysis of cells loaded with <b>Cy7-Gd</b> derivatives, highly hydrophobic and non-anionic dyes were effective for enhancing cell-permeation of Gd<sup>3+</sup> complexes. Furthermore, the behavior of these <b>Cy7-Gd</b> derivatives was examined in mice. Thus, conjugation of hydrophobic fluorescent dyes appears to be an effective approach to improve the cell-permeability of Gd<sup>3+</sup> complexes, and should be applicable for further development of Gd<sup>3+</sup>-based MRI contrast agents. |
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Authors:
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Takehiro Yamane; Kenjiro Hanaoka; Yasuaki Muramatsu; Keita Tamura; Yusuke Adachi; Yasushi Miyashita; Yasunobu Hirata; Tetsuo Nagano |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-11 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: - ISSN: 1520-4812 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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