Document Detail


Methionine sulfoxide reductase A (MsrA) protects cultured mouse embryonic stem cells from H2O2-mediated oxidative stress.
MedLine Citation:
PMID:  20506347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methionine sulfoxide reductase A (MsrA), a member of the Msr gene family, can reduce methionine sulfoxide residues in proteins formed by oxidation of methionine by reactive oxygen species (ROS). Msr is an important protein repair system which can also function to scavenge ROS. Our studies have confirmed the expression of MsrA in mouse embryonic stem cells (ESCs) in culture conditions. A cytosol-located and mitochondria-enriched expression pattern has been observed in these cells. To confirm the protective function of MsrA in ESCs against oxidative stress, a siRNA approach has been used to knockdown MsrA expression in ES cells which showed less resistance than control cells to hydrogen peroxide treatment. Overexpression of MsrA gene products in ES cells showed improved survivability of these cells to hydrogen peroxide treatment. Our results indicate that MsrA plays an important role in cellular defenses against oxidative stress in ESCs. Msr genes may provide a new target in stem cells to increase their survivability during the therapeutic applications.
Authors:
Chi Zhang; Pingping Jia; Yuanyuan Jia; Herbert Weissbach; Keith A Webster; Xupei Huang; Sharon L Lemanski; Mohan Achary; Larry F Lemanski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  111     ISSN:  1097-4644     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2011-02-14     Revised Date:  2012-03-09    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  94-103     Citation Subset:  IM    
Copyright Information:
(c) 2010 Wiley-Liss, Inc.
Affiliation:
Department of Biomedical Science, Florida Atlantic University, Boca Raton, Florida 33431, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Embryonic Stem Cells / cytology,  drug effects*,  physiology*
Humans
Hydrogen Peroxide / pharmacology*
Methionine Sulfoxide Reductases / genetics,  metabolism*
Mice
Oxidants / pharmacology*
Oxidative Stress*
RNA, Small Interfering / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
HL-061246/HL/NHLBI NIH HHS; HL-58435/HL/NHLBI NIH HHS; R01 HL044578-06A2/HL/NHLBI NIH HHS; R01 HL072924-06/HL/NHLBI NIH HHS; R01 HL092499-01A1/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Oxidants; 0/RNA, Small Interfering; 0/Recombinant Fusion Proteins; 7722-84-1/Hydrogen Peroxide; EC 1.8.4.-/Methionine Sulfoxide Reductases
Comments/Corrections

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