| Methionine analogs and cell division regulation in the yeast Saccharomyces cerevisiae. | |
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MedLine Citation:
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PMID: 366609 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methionine analogs such as ethionine, selenomethionine, and trifluoromethionine all arrest growth and division of the yeast Saccharomyces cerevisiae. One analog, ethionine, caused cells of the yeast to arrest specifically within G1; reciprocal shift experiments showed that ethionine and alpha-factor arrested cells at the same step ("start"). The major effect of ethionine on synthesis of macromolecules was to reduce both the rate of appearance of 35S ribosomal precursor RNA and the rate of production of mature rRNA. Synthesis of protein was relatively unaffected by ethionine. Selenomethionine and trifluoromethionine caused cells to arrest randomly in the cell division cycle. Although treatment of cells with either selenomethionine or trifluoromethionine also reduced the rate of total RNA synthesis, each of these analogs had other effects that presumably prohibited completion of the cell cycle. We propose that the rate of rRNA production is an important regulatory event in the cell cycle. |
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Authors:
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R A Singer; G C Johnston; D Bedard |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 75 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1978 Dec |
Date Detail:
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Created Date: 1979-03-13 Completed Date: 1979-03-13 Revised Date: 2010-09-02 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 6083-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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drug effects Cell Division / drug effects* Ethionine / pharmacology* Fungal Proteins / biosynthesis Methionine / analogs & derivatives*, pharmacology Methylation Nucleic Acid Precursors / biosynthesis RNA / biosynthesis RNA, Ribosomal / biosynthesis* RNA, Transfer / biosynthesis Saccharomyces cerevisiae / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Fungal Proteins; 0/Nucleic Acid Precursors; 0/RNA, Ribosomal; 13073-35-3/Ethionine; 63-68-3/Methionine; 63231-63-0/RNA; 9014-25-9/RNA, Transfer |
| Comments/Corrections | |
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