Document Detail


Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein.
MedLine Citation:
PMID:  17055997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recombinant mouse 5,10-methenyltetrahydrofolate synthetase (MTHFS) was expressed in Escherichia coli and shown to co-purify with a chromophore that had a lambda(max) at 320nm. The chromophore remained bound to MTHFS during extensive dialysis, but dissociated from MTHFS when its substrate, 5-formyltetrahydrofolate, was bound. The chromophore was identified as an oxidized catecholamine by mass spectrometry and absorption spectroscopy. Purified recombinant mouse MTHFS and rabbit liver MTHFS proteins were shown to bind oxidized N-acetyldopamine (NADA) tightly. The addition of NADA to cell culture medium accelerated markedly folate turnover and decreased both folate accumulation and total cellular folate concentrations in MCF-7 cells. Expression of the MTHFS cDNA in MCF-7 cells increased the concentration of NADA required to deplete cellular folate. The results of this study are the first to identify a link between catecholamines and one-carbon metabolism and demonstrate that NADA accelerates folate turnover and impairs cellular folate accumulation in MCF-7 cells.
Authors:
Montserrat C Anguera; Patrick J Stover
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-10-04
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  455     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-14     Completed Date:  2007-01-12     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  175-87     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Breast Neoplasms / chemistry*,  metabolism*
Carbon-Nitrogen Ligases / chemistry*,  metabolism*
Catecholamines / chemistry*,  metabolism*
Cell Line, Tumor
Enzyme Activation
Folic Acid / metabolism*
Humans
Mice
Protein Binding
Rabbits
Substrate Specificity
Grant Support
ID/Acronym/Agency:
DK07158-28/DK/NIDDK NIH HHS; HD35687/HD/NICHD NIH HHS; R01 HD035687/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Catecholamines; 935E97BOY8/Folic Acid; EC 6.3.-/Carbon-Nitrogen Ligases; EC 6.3.3.2/5,10-methenyltetrahydrofolate synthetase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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