| Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein. | |
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MedLine Citation:
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PMID: 17055997 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recombinant mouse 5,10-methenyltetrahydrofolate synthetase (MTHFS) was expressed in Escherichia coli and shown to co-purify with a chromophore that had a lambda(max) at 320nm. The chromophore remained bound to MTHFS during extensive dialysis, but dissociated from MTHFS when its substrate, 5-formyltetrahydrofolate, was bound. The chromophore was identified as an oxidized catecholamine by mass spectrometry and absorption spectroscopy. Purified recombinant mouse MTHFS and rabbit liver MTHFS proteins were shown to bind oxidized N-acetyldopamine (NADA) tightly. The addition of NADA to cell culture medium accelerated markedly folate turnover and decreased both folate accumulation and total cellular folate concentrations in MCF-7 cells. Expression of the MTHFS cDNA in MCF-7 cells increased the concentration of NADA required to deplete cellular folate. The results of this study are the first to identify a link between catecholamines and one-carbon metabolism and demonstrate that NADA accelerates folate turnover and impairs cellular folate accumulation in MCF-7 cells. |
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Authors:
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Montserrat C Anguera; Patrick J Stover |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-10-04 |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 455 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-14 Completed Date: 2007-01-12 Revised Date: 2011-05-02 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: United States |
Other Details:
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Languages: eng Pagination: 175-87 Citation Subset: IM |
Affiliation:
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Cornell University, Division of Nutritional Sciences, Ithaca, NY 14853, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Breast Neoplasms / chemistry*, metabolism* Carbon-Nitrogen Ligases / chemistry*, metabolism* Catecholamines / chemistry*, metabolism* Cell Line, Tumor Enzyme Activation Folic Acid / metabolism* Humans Mice Protein Binding Rabbits Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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DK07158-28/DK/NIDDK NIH HHS; HD35687/HD/NICHD NIH HHS; R01 HD035687-09/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Catecholamines; 59-30-3/Folic Acid; EC 6.3.-/Carbon-Nitrogen Ligases; EC 6.3.3.2/5,10-methenyltetrahydrofolate synthetase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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