| Methanosaeta harundinacea sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor. | |
| | |
MedLine Citation:
|
PMID: 16403877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two methanogenic strains, 8AcT and 6Ac, were isolated from an upflow anaerobic sludge blanket reactor treating beer-manufacture wastewater in Beijing, China. Cells of strains 8AcT and 6Ac were rod-shaped (0.8-1.0 x 3-5 microm) and non-motile, occurring singly or in pairs; however, at high cell density the cells were arranged in long chains within a common sheath. The two strains used acetate exclusively for growth and methane production. The specific growth rate of strain 8AcT was 0.030 h(-1) when growing in acetate (20 mM) at 37 degrees C. The temperature range for growth was 25-45 degrees C, with the fastest growth at 34-37 degrees C. The pH range for growth and methane production was 6.5-9.0, with the fastest growth at pH 7.2-7.6. The G+C content of genomic DNA of strain 8AcT was 55.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that the novel strains clustered with Methanosaeta species; the 16S rRNA gene sequence similarities between strain 8AcT and Methanosaeta concilii DSM 3013 and 'Methanosaeta thermophila' DSM 6194 were 92.5 and 87.3 %, respectively. The sequence similarity levels of mcrA, the gene encoding the alpha-subunit of methyl-coenzyme M reductase, and of the deduced amino acids of mcrA, between strain 8AcT and Methanosaeta concilii DSM 3671T were 36 and 78.9 %, respectively. Based on the phylogenetic and phenotypic analyses, the novel species Methanosaeta harundinacea sp. nov. is proposed, with strain 8AcT (= JCM 13211T = CGMCC 1.5026T) as the type strain. |
| | |
Authors:
|
Kai Ma; Xiaoli Liu; Xiuzhu Dong |
Related Documents
:
|
15650687 - Axenic culture of gyrodinium impudicum strain kg03, a marine red-tide microalga that pr... 753397 - Lipid composition of strains of the fungus ustilago zeae (beckm.) differing as regards ... 16347897 - Betaine fermentation and oxidation by marine desulfuromonas strains. 9336917 - Thermosipho melanesiensis sp. nov., a new thermophilic anaerobic bacterium belonging to... 3127657 - A forward mutation assay using ampicillin-resistance in escherichia coli designed for i... 5413827 - Filamentous growth of mucor rouxii under nitrogen. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: International journal of systematic and evolutionary microbiology Volume: 56 ISSN: 1466-5026 ISO Abbreviation: Int. J. Syst. Evol. Microbiol. Publication Date: 2006 Jan |
Date Detail:
|
Created Date: 2006-01-11 Completed Date: 2006-03-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 100899600 Medline TA: Int J Syst Evol Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 127-31 Citation Subset: IM |
Affiliation:
|
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/AY817738; AY970347; AY970348; AY970349 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetates
/
metabolism Anaerobiosis Base Composition Beer Bioreactors* China Culture Media DNA Restriction Enzymes / genetics Hydrogen-Ion Concentration Methane / metabolism Methanosarcinaceae / chemistry, classification*, isolation & purification, physiology Molecular Sequence Data Oxidoreductases / genetics, metabolism RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Homology, Nucleic Acid Sewage / microbiology* Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Acetates; 0/Culture Media; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/Sewage; 74-82-8/Methane; EC 1.-/Oxidoreductases; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/endodeoxyribonuclease McrA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Polyphasic study of Zymomonas mobilis strains revealing the existence of a novel subspecies Z. mobil...
Next Document: rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions...