| Methanol oxidation and assimilation in Hansenula polymorpha. An analysis by 13C n.m.r. in vivo. | |
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MedLine Citation:
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PMID: 1747123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of methanol was monitored in whole cells of the methylotrophic yeast Hansenula polymorpha by using [13C]methanol and n.m.r. in vivo. The main products observed under normal conditions were trehalose and glycerol, whereas cells that were starved before exposure to [13C]methanol also accumulated glutamate, glutamine and alanine; formate was also more prominent in spectra from starved cells. Cells exposed to high methanol concentration together with high oxygenation oxidized methanol extensively, leading to formaldehyde accumulation; label was not found in any subsequent metabolic products, indicating possible cell inactivation. [13C]Formate was incorporated into metabolic products in glucose-grown cells exposed to 150 mM-methanol for 3 h, but not in cells starved for 3 h, in which it was oxidized. At 21 degrees C such 3 h-starved cells showed a slower metabolism of [13C]methanol compared with those at 37 degrees C, and also converted methanol into formate rather than into assimilation products. The labelling pattern in trehalose from starved cells at 37 degrees C was consistent with methanol assimilation via the pentose phosphate pathway. Lack of appearance of labelled formaldehyde and formate during metabolism under normal conditions suggests that the linear oxidation pathway is not a major contributor to methanol oxidation; their appearance in extreme conditions suggests instead a more likely role in detoxification. |
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Authors:
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J G Jones; E Bellion |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 280 ( Pt 2) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1992-01-15 Completed Date: 1992-01-15 Revised Date: 2010-09-09 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 475-81 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Texas, Arlington 76019-0065. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbon Isotopes Formic Acids / metabolism Magnetic Resonance Spectroscopy Methanol / metabolism* Oxidation-Reduction Pichia / growth & development, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM-38571/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 0/Formic Acids; 64-18-6/formic acid; 67-56-1/Methanol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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