| Methanol-acetic anhydride: an efficient blocking agent for electron microscope cytochemistry. Its application to mouse testis and other tissues. | |
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MedLine Citation:
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PMID: 6186647 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A mixture of absolute methanol and acetic anhydride (MA) (5:1, v/v; 24 h at 25 degrees C) which is known to methylate and acetylate--respectively--free carboxyl and amino groups in proteins, was tested for its effectiveness as a blocking agent in glutaraldehyde-fixed mouse testis and skeletal muscle. In young spermatids the staining of the acrosome with either uranyl acetate (UA) or ethanolic phosphotungstic acid (PTA) was completely prevented by prior treatment with MA. Esterification of carboxyl groups with 0.1 N HCl in methanol (24 h at 30 degrees C) eliminated the UA staining without affecting that due to PTA. It is concluded that--COOH groups are responsible for UA binding and that acetylation (of amino groups) prevented PTA binding. MA also abolishes the strong affinity of PTA to the lateral elements of the synaptonemal complex in meiotic chromosomes, the axoneme and fibrous sheath of the spermatid tail and the Z band in skeletal muscle. Reactivity was diminished in nucleoli and remained unaffected in chromatin, the outer coarse fibers of the flagellum and collagen fibrils. Different functional groups may participate in PTA staining. The ultrastructure was well preserved in all cases. |
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Authors:
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C J Tandler; A J Solari |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Histochemistry Volume: 76 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1982 |
Date Detail:
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Created Date: 1983-04-21 Completed Date: 1983-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 351-61 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acids
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diagnostic use* Acetic Anhydrides / diagnostic use* Acetylation Animals Crithidia / analysis, ultrastructure Histocytochemistry / methods* Indicators and Reagents Male Methanol / diagnostic use* Methylation Mice Microscopy, Electron Staining and Labeling Testis / analysis*, ultrastructure Trypanosoma cruzi / analysis, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Acetic Acids; 0/Acetic Anhydrides; 0/Indicators and Reagents; 67-56-1/Methanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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