Document Detail

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri.
MedLine Citation:
PMID:  6480559     Owner:  NLM     Status:  MEDLINE    
Cell lysates of acetate-grown Methanosarcina barkeri 227 were found to cleave acetate to CH4 and CO2. The aceticlastic reaction was identified by using radioactive methyl-labeled acetate. Cell lysates decarboxylated acetate in a nitrogen atmosphere, conserving the methyl group in methane. The rate of methanogenesis from acetate in the cell lysates was comparable to that observed with whole cells. Aceticlastic activity was found in the particulate fraction seperate from methylcoenzyme M methylreductase activity, which occurs in the soluble fraction. Pronase treatment eliminated methylcoenzyme M methylreductase activity in lysates and stimulated aceticlastic activity, indicating the aceticlastic activity was not derived from unbroken cells, which are unaffected by proteolytic treatment.
L Baresi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  160     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1984 Oct 
Date Detail:
Created Date:  1984-11-15     Completed Date:  1984-11-15     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  365-70     Citation Subset:  IM    
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MeSH Terms
Acetates / metabolism*
Carbon Radioisotopes / diagnostic use
Cell-Free System
Euryarchaeota / metabolism*
Methane / metabolism
Species Specificity
Subcellular Fractions / metabolism
Reg. No./Substance:
0/Acetates; 0/Carbon Radioisotopes; 74-82-8/Methane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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