Document Detail

Metformin restores the penile expression of nitric oxide synthase in high-fat-fed obese rats.
MedLine Citation:
PMID:  17314235     Owner:  NLM     Status:  MEDLINE    
Obesity is a well-known risk factor for erectile dysfunction, which is associated with reduced penile nitric oxide synthase (NOS) expression. Recently it was reported that metformin activates AMP-activated protein kinase (AMPK), which increases the expression of neuronal (n) NOS and endothelial (e) NOS. Thus, to evaluate whether metformin restores NOS expression in penile tissue, we measured penile expression of nNOS and eNOS after 4 weeks of metformin treatment (300 mg/kg/d) in 5-month-old high-fat-fed obese (HFO) rats. HFO rats have increased fat accumulation in visceral areas and marked suppression of nNOS and eNOS expression in penile tissue. However, metformin treatment decreased visceral fat deposition and restored nNOS and eNOS expression in penile tissue. The levels of AMPK and phosphorylated AMPK were also decreased in HFO rats but were subsequently elevated by metformin treatment. These results suggest that expression of NOS was suppressed by the high-fat diet but restored by metformin treatment. The effect of metformin on the expression of NOS may be associated with its activation of AMPK.
Yong-Woon Kim; So-Young Park; Jong-Yeon Kim; Jung-Yoon Huh; Woo-Sung Jeon; Chang-Jun Yoon; Sung-Su Yun; Ki-Hak Moon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-21
Journal Detail:
Title:  Journal of andrology     Volume:  28     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    2007 Jul-Aug
Date Detail:
Created Date:  2007-06-25     Completed Date:  2007-10-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  United States    
Other Details:
Languages:  eng     Pagination:  555-60     Citation Subset:  IM    
Department of Urology, College of Medicine, Yeungnam University, 317-1 Daemyung-Dong, Nam-Gu, Daegu 705-035, Republic of Korea.
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MeSH Terms
Adenylate Kinase / blood
Adipose Tissue / anatomy & histology
Body Weight
Dietary Fats*
Metformin / therapeutic use*
Nitric Oxide Synthase / genetics*
Nitric Oxide Synthase Type III / genetics
Obesity / enzymology*,  etiology
Organ Size
Penis / enzymology*
Rats, Mutant Strains
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Dietary Fats; 657-24-9/Metformin; EC Oxide Synthase; EC Oxide Synthase Type III; EC Kinase

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