| Metastatic adenocarcinoma to the brain: an immunohistochemical approach. | |
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MedLine Citation:
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PMID: 9269830 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The diagnosis of metastatic adenocarcinoma to the brain of unknown primary is problematic, and the role of immunohistochemistry in identifying a source has not been fully characterized. Sixty-eight metastatic adenocarcinomas of the brain with known primaries were immunostained with antibodies to cytokeratin 7 (CK7), cytokeratin 20 (CK20), CAM 5.2, wide-spectrum keratin (WSK), gross cystic disease fluid protein-15 (GCDFP-15), glial fibrillary acidic protein (GFAP), estrogen receptor (ER), and progesterone receptor (PR). True positive staining was defined as CK7 in lung or breast; CK20 in gastrointestinal; and GCDFP-15, ER, and PR in breast carcinomas. CK7 immunoreactivity was present in all 27 lung carcinomas and 14 of 15 breast carcinomas with a sensitivity of 98% and specificity of 78%. CK20 stained 15 of 16 GI carcinomas with a sensitivity and specificity of 94%. None of the cytokeratins stained surrounding brain tissue. GCDFP-15, ER, and PR had sensitivities of 33%, 33%, and 87%, with specificities of 92%, 84%, and 28%, respectively. PR often stained nuclei of normal brain tissue and was accentuated in areas of necrosis or cautery artifact. CK7 and CK20 are highly sensitive and specific in patients with metastatic adenocarcinoma to the brain of unknown primary. GCDFP-15 and ER are relatively specific, but insensitive markers, and PR is nonspecific and difficult to interpret. |
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Authors:
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A Perry; J E Parisi; P J Kurtin |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Human pathology Volume: 28 ISSN: 0046-8177 ISO Abbreviation: Hum. Pathol. Publication Date: 1997 Aug |
Date Detail:
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Created Date: 1997-09-18 Completed Date: 1997-09-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421547 Medline TA: Hum Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 938-43 Citation Subset: IM |
Affiliation:
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Department of Anatomic Pathology, Mayo Clinic, Rochester, MN 55905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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chemistry*,
secondary* Apolipoproteins* Apolipoproteins D Brain Neoplasms / chemistry*, secondary* Carrier Proteins / analysis Glial Fibrillary Acidic Protein / analysis Glycoproteins* Humans Immunohistochemistry Intermediate Filament Proteins / analysis Keratin-20 Keratins / analysis Membrane Transport Proteins* Neoplasms, Unknown Primary / diagnosis* Receptors, Estrogen / analysis Receptors, Progesterone / analysis Tumor Markers, Biological / analysis* |
| Chemical | |
Reg. No./Substance:
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0/APOD protein, human; 0/Apolipoproteins; 0/Apolipoproteins D; 0/Carrier Proteins; 0/Glial Fibrillary Acidic Protein; 0/Glycoproteins; 0/Intermediate Filament Proteins; 0/KRT20 protein, human; 0/Keratin-20; 0/Membrane Transport Proteins; 0/PIP protein, human; 0/Receptors, Estrogen; 0/Receptors, Progesterone; 0/Tumor Markers, Biological; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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