Document Detail

Metastasis-focused cell-based SELEX generates aptamers inhibiting cell migration and invasion.
MedLine Citation:
PMID:  20473891     Owner:  NLM     Status:  MEDLINE    
Metastasis, the capacity of tumour cells to disseminate and grow at distant sites, is the main factor in cancer mortality. Compounds inhibiting migration and invasion of cancer cells are promising candidates for anticancer therapy strategies. We have generated nuclease-resistant RNA ligands (aptamers) recognizing highly metastatic cells with high affinity and specificity, and inhibiting their migratory and invasive potentials. Aptamers were generated by a cell-based subtractive SELEX technology using isogenic cell lines with similar tumorigenic potentials but opposite metastatic aggressiveness. Two aptamers, E37 and E10, bound specifically to the metastatically aggressive cell line and altered the phosphorylation of several tyrosine kinases. Fluorescent microscopy showed intracellular uptake of E37, in contrast to membrane binding of E10. Both aptamers inhibited migration of tumour cells in culture (50 and 85% inhibition with respect to control pool for E10 and E37, respectively) while only E10 inhibited cell invasion (-75% with respect to control pool). This proof-of-concept study demonstrates the potential of cell-based SELEX to yield ligands that selectively recognize aggressive metastatic cells and inhibit phenotypes linked to metastatic potential.
Elina Zueva; Laila Illán Rubio; Frédéric Ducongé; Bertrand Tavitian
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  128     ISSN:  1097-0215     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2010-12-24     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  797-804     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 UICC.
Inserm, U1023, 91400 Orsay, France.
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MeSH Terms
Aptamers, Nucleotide / therapeutic use*
Blotting, Western
Breast Neoplasms / genetics,  pathology*,  prevention & control*
Cell Adhesion
Cell Line, Transformed
Cell Movement*
Embryo, Mammalian / cytology,  metabolism
Fibroblasts / cytology,  metabolism
NIH 3T3 Cells
Neoplasm Invasiveness
SELEX Aptamer Technique*
Wound Healing
Reg. No./Substance:
0/Aptamers, Nucleotide

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