Document Detail

Metaproteome analysis and molecular genetics of rat intestinal microbiota reveals section and localisation resolved species distribution and enzymatic functionalities.
MedLine Citation:
PMID:  23016992     Owner:  NLM     Status:  Publisher    
The digestion of food ingredients depends on the action of the gut microbiota and has a significant influence on the health, especially in case of metabolic diseases of the host organism. Despite the relevance of the structure and functionalities in the microbiota for the metabolism of the host the spatial resolution of microbial consortia and the functionalities in the different gut sections of rat are mostly unknown. Since there are suitable rat models for human metabolic diseases the microbiota of the rat is of special interest. Samples along the intestinal tract of rats were investigated using metaproteomics and 16S rRNA gene pyrosequencing. The procedures for harvesting bacteria from the mucus and the content of the gut sections and feces were optimized leading to 2,802 non-redundant bacterial protein groups in total that were assigned to spectra measured by liquid chromatography-tandem mass spectrometry. The majority of 16S rRNA genes and protein groups belonged to members of the Firmicutes, Bacteroidetes and Proteobacteria. The functionalities in the enzyme repertoire were compared between the mucus and the content of the large intestine sections and the feces samples. This spatial resolution allowed pinpointing changes in the community to specific metabolic capacities like carbohydrate transport and energy conservation. The results showed that the mere analysis of feces samples reflect the functions of the gut microbiota only to a minor extend and sheds light on the metabolic interchange between the microbiota and the host organism.
Sven-Bastiaan Haange; Andreas Oberbach; Nadine Schlichting; Floor Hugenholtz; Hauke Smidt; Martin von Bergen; Holger Till; Jana Seifert
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-27
Journal Detail:
Title:  Journal of proteome research     Volume:  -     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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