Document Detail

Metaphase arrest and delay in cell cycle kinetics of root apical meristems and mouse bone marrow cells treated with leaf aqueous extract of Clerodendrum viscosum Vent.
MedLine Citation:
PMID:  23294356     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVES: To study cell cycle delay and metaphase arresting activity of leaf aqueous extract of Clerodendrum viscosum Vent. (LAECV) in root apical meristems and mouse bone marrow cells.
MATERIALS AND METHODS: Cell cycle delay and metaphase arresting activities of LAECV were analysed, in root apical meristems of onion and wheat, and in mouse bone marrow cells, by scoring mitotic index, metaphase frequency and transition of cells from metaphase to anaphase. Colchicine was used as the standard metaphase arresting drug. Phytochemicals present in LAECV were detected and their phytotoxic activity was evaluated by analysing green-gram (Vigna radiata) seedling's root growth retardation and branch root swelling phenomenon.
RESULTS: LAECV treatment resulted in dose-dependent root growth retardation of green-gram seedling root length (P < 0.01) and half maximal growth inhibitory concentration (IC(50) ) of LAECV was 0.87 mg/ml at 144 h. In onion and wheat root meristem cells the mitotic index decreased, metaphase frequency increased and transition from metaphase to anaphase reduced. Experimentation with mouse bone marrow cells indicated that LAECV induced metaphase arrest (164.3% increase in arrested metaphases per 300 mg/kg body weight, over 2.5 h). Phytochemicals like carbohydrates, glycosides, saponins, terpenoids, triterpenoids, tannins and trace amounts of alkaloids were detected in LAECV.
CONCLUSION: It may be said that LAECV contains mitostatic and metaphase arresting components that are able to induce significant metaphase arrest in root apical meristems and also in mouse bone marrow cells.
S Ray; L M Kundu; S Goswami; G C Roy; S Chatterjee; S Dutta; A Chaudhuri; C S Chakrabarti
Related Documents :
23149076 - Ecdysone-dependent and ecdysone-independent programmed cell death in the developing opt...
23505386 - Both the caspase csp-1 and a caspase-independent pathway promote programmed cell death ...
22944296 - Epithelial and cartilaginous differentiation in clear cell chondrosarcoma.
24985206 - Arginine-glycine-glutamine and serine-isoleucine-lysine-valine-alanine-valine modified ...
21796416 - Synergistic interaction between sunitinib and docetaxel is sequence dependent in human ...
25162916 - Bioactive natural constituents from lemongrass tea and erythropoiesis boosting effects:...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell proliferation     Volume:  46     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  109-17     Citation Subset:  IM    
Copyright Information:
© 2013 Blackwell Publishing Ltd.
Molecular Biology and Genetics Unit, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In silico analysis of molecular mechanisms of legume lectin-induced apoptosis in cancer cells.
Next Document:  Presenting D-dimer and early symptom severity are independent predictors for post-thrombotic syndrom...